It is possible to produce PBL's from leaf tissue from a stem prop. I have seen discussions, someone had a paper, and a friend tried it. He had good initial success but was got busy and wasn't able to complete the try. I think he grew the leaf on regular media like O139 from Phytotech. The difference was the hormone used and I don't know what that was. It is fairly toxic.
If you don't want many plant, I'd just stick with stem props. Much easier. This guy wanted to produce the PBL's so that maybe he could colchicine treat them and make tetraploid versions of the mother plant.
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