Is there a 100% homemade media recipe that doen't require replating?
Login
User Name
Password   


Registration is FREE. Click to become a member of OrchidBoard community
(You're NOT logged in)

menu menu

Sponsor
Donate Now
and become
Forum Supporter.

Is there a 100% homemade media recipe that doen't require replating?
Many perks!
<...more...>


Sponsor
 

Google


Fauna Top Sites
LOG IN/REGISTER TO CLOSE THIS ADVERTISEMENT
Go Back   Orchid Board - Most Complete Orchid Forum on the web ! > >
Reply
 
Thread Tools Rate Thread Display Modes
  #1  
Old 02-25-2009, 10:44 AM
altcuban altcuban is offline
Jr. Member
 

Join Date: Feb 2009
Posts: 4
Is there a 100% homemade media recipe that doen't require replating?
Default Is there a 100% homemade media recipe that doen't require replating?

Hello guys I want to try pollinating and propagating a phal, I've read a lot of articles and posts and I'm worried about the media. I want to use a media I can make at home without having to buy it online. I know I will need to buy the agar and I have 20-20-20 and root hormone already. So is there any receipe for media that doesn't require a medium powder, just the normal stuff I have at home + the agar.
Also, I'm afraid about contamination when replating is there a media recipe I can use that will allow me to not have to reflask, one where the seeds can germinate and the seedlings grow until mature enough for deflasking. In other words a media that works for both so there is no need to replate. I know i will have to open a ventilation.

Thanks for your help.
Reply With Quote
  #2  
Old 02-25-2009, 11:22 AM
dabblin-n-orchids dabblin-n-orchids is offline
Senior Member
 

Join Date: Jan 2009
Posts: 134
Is there a 100% homemade media recipe that doen't require replating?
Default

I have never flasked before but i found this when i was researching it online:
DIY - Guide to orchid propagation - Orchid Article Library
oh and some people have used gelatin as a gelling agent instead of agar
Reply With Quote
  #3  
Old 02-25-2009, 11:28 AM
John D. John D. is offline
Senior Member
 

Join Date: Nov 2007
Zone: 7a
Location: Powhatan VA
Posts: 190
Default

OSP1 is a formula developed by the Orchid seedbank project (Aaron Hicks) cited 1999 as a simple medium for germination of orchids at home using readily available items. For 1 liter add sugar (cane sugar from the grocery store) 2 level teaspoons, Miracle grow fertilizer 1/2 level teaspoon, bananas (from baby food no sugar added) 150 ml. ;stir to suspend or dissolve and dispense to culture jars and sterilize by autoclaving (pressure cooking). He puts a circle of filterpaper in the culture jar to support the seeds as this is a liquid medium. If you add about eight grams of agar you will have a solid medium. If you use agar add it to the large amount of medium and bring it to a rolling boil for 1-2 minutes to dissolve the agar before pouring the smaller vessels, keep stirring to keep the agar mixed well as you pour so it will by evenly mixed in the smaller vessels.
Your 20-20-20 can be substituted for the MG if it has micronutrients.
For Phals I would cut it back to 80% (Add an extra 250 ml of water)instead of baby food you can use 3 tablespoons regular banana, try adding potato 6 tablespoons in addition to the banana.
Replating is to prevent overcrowding so don't put much seed in a flask.

Last edited by John D.; 02-25-2009 at 11:32 AM..
Reply With Quote
  #4  
Old 02-26-2009, 10:09 AM
altcuban altcuban is offline
Jr. Member
 

Join Date: Feb 2009
Posts: 4
Is there a 100% homemade media recipe that doen't require replating?
Default thanks

Thank you very much guys, you've been extra helpful
Reply With Quote
  #5  
Old 02-26-2009, 10:19 AM
Royal Royal is offline
Senior Member
 

Join Date: Jan 2008
Zone: 8a
Location: Piney Woods of East Texas
Age: 44
Posts: 3,253
Default

Yeah, that's a cool recipe. Thanks for posting it JohnD. I've only used prepared media until now, but now that I've got a few things down I might try making my own just for kicks. Do you use this one?

altcuban, Much depends on what species you are growing. Some just take a while and may need at least one replate. Some grow up just fine in the mother flask. Not crowding is crucial.
Reply With Quote
  #6  
Old 02-26-2009, 02:33 PM
kinknstein kinknstein is offline
Senior Member
 

Join Date: Oct 2008
Zone: 3b
Location: Edmonton Alberta
Age: 40
Posts: 1,484
Default

This is great info!
Reply With Quote
  #7  
Old 02-26-2009, 07:16 PM
John D. John D. is offline
Senior Member
 

Join Date: Nov 2007
Zone: 7a
Location: Powhatan VA
Posts: 190
Default

I am using mostly Phytotech 658 for germination and with the banana and potato added for replates. I have used close to the OSP1 for liquid medium for protocorm conversion to 4N.
I am going to try some with agar for regular germination when I have some extra time and seed.
Reply With Quote
  #8  
Old 02-26-2009, 07:25 PM
newflasker newflasker is offline
Senior Member
 

Join Date: Jun 2007
Location: Virginia
Posts: 153
Is there a 100% homemade media recipe that doen't require replating?
Default

The simple media formula for sowing Phal that you can find chemicals in your house or in your local area:
- 1 gram 20-10-20 fertilizer (no urea)
- 20 gram sugar
- 7 gram agar
That it's. Mix in hot water to make a litter of solution then poor into small jars; autoclave; let them cool then drop your seeds in. If you want to use this media then you need to replate your seedlings every 3 - 4 months because pH will drop and seedlings grow slow. That is a problem of home-made media. In commercial media, there are many additional chemicals that help seedlings grow fast: extremely fine activated charcoal to adsorb acid and bad substances such as phenolic; NAA + BAP improve root and leave; inositol to enhance cell grow; MES to keep pH not change... So if you want to grow for fun and get so so seedlings then use it other than that get commercial medium. A picture of Phal seeds in the above medium. Cheers and good luck.
Attached Thumbnails
Is there a 100% homemade media recipe that doen't require replating?-germinate-jpg  
Reply With Quote
  #9  
Old 02-27-2009, 08:06 PM
10010100102 10010100102 is offline
Senior Member
 

Join Date: Jan 2009
Location: Ontario
Posts: 165
Default

I'm also using the fert + sugar + agar recipie, but personally I use regular KNOX brand gelatin instead of agar. I can't tell you if this works better or worse than agar as I've never actually tried agar, but I've very definately been able to germinate some Phal. seeds with it.

I've read up on a few different recipies, and one thing I'm experimenting with now is adding a multivitamin to the medium for extra nutrients.

Hope this helps and good luck!
Reply With Quote
  #10  
Old 03-01-2009, 10:15 AM
Royal Royal is offline
Senior Member
 

Join Date: Jan 2008
Zone: 8a
Location: Piney Woods of East Texas
Age: 44
Posts: 3,253
Default

Quote:
Originally Posted by 10010100102 View Post
I'm also using the fert + sugar + agar recipie, but personally I use regular KNOX brand gelatin instead of agar. I can't tell you if this works better or worse than agar as I've never actually tried agar, but I've very definately been able to germinate some Phal. seeds with it.
Can't argue with results. Another cool tip. How much Knox do you use per liter?
Reply With Quote
Reply

Bookmarks

Tags
homemade media, homemade medium, media, no replate, no replate needed, doent, require, replating, recipe, 100%, homemade


Currently Active Users Viewing This Thread: 1 (0 members and 1 guests)
 
Thread Tools
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off


Similar Threads
Thread Thread Starter Forum Replies Last Post
Anyone have a homemade insecticide recipe? sammich Pests & Diseases 4 06-22-2008 12:54 PM

All times are GMT -4. The time now is 08:33 PM.

© 2007 OrchidBoard.com
Search Engine Optimisation provided by DragonByte SEO v2.0.37 (Lite) - vBulletin Mods & Addons Copyright © 2021 DragonByte Technologies Ltd.
Feedback Buttons provided by Advanced Post Thanks / Like (Lite) - vBulletin Mods & Addons Copyright © 2021 DragonByte Technologies Ltd.

Clubs vBulletin Plugins by Drive Thru Online, Inc.