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-   -   Flask Shelf life (https://www.orchidboard.com/community/propagation/60729-flask-shelf-life.html)

Carey_K 06-27-2012 01:56 AM

Flask Shelf life
 
Let's assume that I am going to be able to flask correctly in a contaminant free environment. I leave the flasks in the fridge for five days to check for contamination & spore formation etc.. My question is how long can a contaminant free flask stay that way before sowing seeds into it?:evil:

Gage 06-27-2012 09:42 AM

From what I have read, the longer it goes without growing anything, the more certain you can be that it is contaminant free. But the outside of the flask would need to be cleaned/sterilized before working with it.

zxyqu 06-27-2012 10:06 AM

They are useable for quite awhile, as the plants are growing in them for 6+months anyway. But I'd try to use them in 1-3 months are making them, with 3 being a stretch. Also, if you want to check for contaminants, you probably shouldn't test in a fridge. Put it in your grow space. Then you'll find out if you were really contaminant free, and this is where they'll be placed anyway to grow plantlets.

camille1585 06-27-2012 10:07 AM

I would also leave the flasks outside the fridge, you'll spot any contamination more quickly. Why did you put them there?

DavidCampen 06-27-2012 01:18 PM

Since I have never germinated orchid seeds but I have germinated mushroom spores, allow me to ask some silly questions. These are agar flasks right? Weren't they prepared with a pressure cooker? Why would newly prepared flasks have any viable contaminants?

zxyqu 06-27-2012 01:21 PM

Totally reasonable question David, but it depends on if someone used a pressure cooker (some people boil in a microwave, or use Bleach with some success). Also, another key factor is making sure they stay contaminant free following sowing, though that may be beyond the scope of OP and your post.


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