Butterwort Tissue Culture

[Dorchid]

Working on propagating Mexican Butterwort via tissue culture. This is Pinguicula Moranensis J. 2 weeks in from sterilized explants (carnivorous leaves and meristem). The leaves have yet to show any signs of striking and the meristem died but the 2 leaves encapsulating the meristem have started to grow. They were whitish pink when I plated them but have now started to unfurl and turn green. Unfortunately, I didn’t snap a pic when they were plated. These 2 pics represent 4 days growth between them. Hopefully, they will form calluses and begin to generate plantlets in the near future.

[Dorchid]

Starting to see some plantlets develop.

[Louis_W]

Cool!

So that gel has nutrients as well as growth stimulants and rooting hormones? I guess I don't know much about the nitty gritty of meristem cloning...

[Dorchid]

Yep. This medium is Linsmaier and Skoog basal medium which was developed in 1964. It contains the macro and micronutrients, vitamins and sucrose needed for in-vitro plant development and is kind of an all purpose blend for a variety of plants. It’s too hot for carnivorous plants so the first run was half strength and the second batch was 20% which I just replated to. I also added a couple synthetic hormones/plant growth regulators, BA and NAA in ratios that promoted the most shoot development and plant weight based on a paper I read. They’re super potent and only used .2mg/L and .1mg/L of medium, respectively.