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  #131  
Old 04-15-2021, 11:10 PM
Draikan Draikan is offline
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So... yet another project??
Haha, don't encourage me. I need to be working on the humidity sensing and environmental control.

We screwed up the aperture settings on those photos which is why the depth of field was giving us trouble. I didn't realize the camera wouldn't "preview" the depth of field on the screen, you have to actually take the photo to see how the depth of field will turn out.

Once we got the aperture figured out the depth of field was way more forgiving, but the photos were getting really dark. I'll post a few with the new settings in just a moment. They have much better focus but I'm using a 5 second exposure now so even the tiniest wiggle causes some blur.

---------- Post added at 08:10 PM ---------- Previous post was at 08:05 PM ----------

Here is a shot with better focus:



And now it's obvious that I need to clean my lens...
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  #132  
Old 04-15-2021, 11:21 PM
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Roberta Roberta is online now
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And now it's obvious that I need to clean my lens...
That last one is quite good - you really are pushing the limits. "Too dark" is generally pretty easy to fix with any photo-editing software, since the detail is all there. ("Too bright", blown highlights, loses data and so not necessarily repairable) If you're not familiar with Irfanview, it is a terrific little photo editor, free for individual use. (Price is VERY right!)
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  #133  
Old 04-16-2021, 12:09 AM
Draikan Draikan is offline
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Thanks Roberta!

Maybe someone who is more familiar with the biology than me can step in, but can we see the individual cells in that last image? There are more than 10 of those spots per mm, which would put their diameter at <100um. I think that's the right range for single cells?

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  #134  
Old 04-16-2021, 12:28 AM
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Wow, that's some crazy photography work!!
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