Mold in Flask
Login
User Name
Password   


Registration is FREE. Click to become a member of OrchidBoard community
(You're NOT logged in)

menu menu

Sponsor
Donate Now
and become
Forum Supporter.

Mold in Flask
Many perks!
<...more...>


Sponsor
 

Google


Fauna Top Sites
LOG IN/REGISTER TO CLOSE THIS ADVERTISEMENT
  #1  
Old 06-06-2018, 11:10 AM
jblume1127 jblume1127 is offline
Jr. Member
 

Join Date: Jun 2018
Posts: 2
Mold in Flask
Default Mold in Flask

Hi, I'm a recent graduate from college and we were able to flask orchids for one of my classes. I know the basic process, but don't have any of the equipment. At the end of the year, I took two flasks home. The phalaenopsis flask looks fine, but there is another orchid species we propagated that I do not know what it is and there is mold growing in the agar.

The tallest seedling is about a half inch tall with minimal roots. The mold is white and has grown pretty steadily for several weeks and now covers the surface of the agar. The taller seedlings seem to be unaffected. Some of the smaller orchids have died and are brown and slimy looking. I'm assuming that's a bad sign, but I'm wondering if I could salvage these guys some way. I was really hoping to keep the new species, being that I have about 16 phals as it is.

I read from this source and others that sometimes the mold isn't a problem depending on the type, but I think in each of those cases, the orchids were a lot larger.

Any help would be appreciated. If I need to put them down, it would be better to know for sure from people more experienced. Thank you!
Attached Thumbnails
Mold in Flask-img_2681-jpg   Mold in Flask-img_2682-jpg   Mold in Flask-img_2683-jpg   Mold in Flask-img_2684-jpg   Mold in Flask-img_2685-jpg  

Reply With Quote
  #2  
Old 06-06-2018, 12:42 PM
Ray's Avatar
Ray Ray is offline
Senior Member
 

Join Date: May 2005
Location: Oak Island NC
Posts: 8,926
Mold in Flask
Default

Mold in a potting medium and mold in a flask are likely different critters.

I have no idea how well it would work, but putting a little bit of Inocucor Garden Solution in the flask, then reclosing it and swirling it around to wet all surfaces might kill the pathogen and boost the plant.
__________________
Ray Barkalow, Orchid Iconoclast
KelpMax™, Inocucor, K-Lite - Working together to grow better orchids.: shop.firstrays.com
Free Shipping in the US! (see terms & conditions for details)

Last edited by Ray; 06-14-2018 at 06:37 AM..
Reply With Quote
  #3  
Old 06-06-2018, 02:47 PM
dansyr dansyr is offline
Member
 

Join Date: Jul 2017
Zone: 6b
Location: Boston
Posts: 32
Mold in Flask Male
Default

I've only had this issue once for seedlings this developed, but the only ones I was able to rescue were ones I emergency deflasked, gave a quick light bleaching, and then rinsed and kept as dry as safely possible. Heavy losses but a couple survived. Most lost all their roots (fungus had already infected) but were able to rebound.

If you try Ray's approach, I'm interpreting this as applying Inocucor directly to flask, I'm very interested to hear how this goes. Would be pretty cool if you could have Inocucor balance out a rampant infection.

Alternatively, I might suggest deflasking, followed by a mild bleach soak (~5-10% for a couple minutes), rinsing well, and then applying Inocucor prior to compotting.
Reply With Quote
  #4  
Old 06-06-2018, 06:12 PM
Orchid Whisperer's Avatar
Orchid Whisperer Orchid Whisperer is offline
Senior Member
 

Join Date: Jul 2012
Zone: 7b
Posts: 2,665
Mold in Flask Male
Default

Without equipment to properly re-sterilize, I would suggest de-flasking, thoroughly rinsing agar off the survivors. Gentle spray of room temperature water with plants in a fine colander.

Initially, I would separate the best and largest plants from the others. Get a shallow plastic container with a clear lid, spread the tiny plants on the bottom of the container (no medium). Mist very lightly, leave the lid open briefly the first day, a little longer on following days. You want that container to be humid inside, but not soaking wet. Inspect the seedlings daily for signs of mold, rinse again as needed.

You can do the same thing, with separate containers, for groups of remaining seedling in progressively worse shape.

Eventually you will get small plants that stay mold free. Transfer the seedlings to shallow pots with fine bark, mist just enough to moisten the bark,also in covered cotainers. You are out of the woods, mostly, when seedlings stop dying and roots and leaves start growing.

This has worked for me (seedlings a bit bigger)
__________________
“The good thing about science is that it's true whether or not you believe in it.”

― Neil deGrasse Tyson

"We know the Earth is not flat because, if it was, cats would have pushed everything off the edge of it by now."
Reply With Quote
  #5  
Old 06-13-2018, 12:15 PM
jblume1127 jblume1127 is offline
Jr. Member
 

Join Date: Jun 2018
Posts: 2
Mold in Flask
Default

Thank you everyone! Turns out the flask only had 3 seedlings and although they look pretty healthy (but small), I'm not holding out much hope for survivors. I will keep everyone updated if there's a miracle.
Reply With Quote
Post Thanks / Like - 1 Likes
Likes Orchid Whisperer liked this post
  #6  
Old 06-13-2018, 05:14 PM
Orchid Whisperer's Avatar
Orchid Whisperer Orchid Whisperer is offline
Senior Member
 

Join Date: Jul 2012
Zone: 7b
Posts: 2,665
Mold in Flask Male
Default

Quote:
Originally Posted by jblume1127 View Post
Thank you everyone! Turns out the flask only had 3 seedlings and although they look pretty healthy (but small), I'm not holding out much hope for survivors. I will keep everyone updated if there's a miracle.
Good luck, I bet you'll get a couple survivors, maybe all three!
__________________
“The good thing about science is that it's true whether or not you believe in it.”

― Neil deGrasse Tyson

"We know the Earth is not flat because, if it was, cats would have pushed everything off the edge of it by now."
Reply With Quote
  #7  
Old 06-13-2018, 11:03 PM
estación seca's Avatar
estación seca estación seca is offline
Senior Member
Mold in Flask
 

Join Date: Jun 2015
Zone: 9b
Location: Phoenix AZ - Lower Sonoran Desert
Posts: 8,911
Mold in Flask Male
Default

In the future, people here have written of focusing sun with a magnifying glass through the flask wall to burn mold at an early stage.
__________________
It's a dry heat.
Reply With Quote
  #8  
Old 06-20-2018, 08:18 PM
Fairorchids's Avatar
Fairorchids Fairorchids is offline
Senior Member
 

Join Date: Aug 2013
Zone: 6a
Location: Maplewood, NJ
Posts: 1,593
Mold in Flask Male
Default

Yep. That was my dad's trick. Works like a charm.
__________________
Kim (Fair Orchids)

Founder of SPCOP (Society to Prevention of Cruelty to Orchid People), with the goal of barring the taxonomists from tinkering with established genera!

I am neither a 'lumper' nor a 'splitter', but I refuse to re-write millions of labels.
Reply With Quote
Reply

Bookmarks

Tags
agar, flask, mold, orchids, species


Currently Active Users Viewing This Thread: 1 (0 members and 1 guests)
 
Thread Tools
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off


Similar Threads
Thread Thread Starter Forum Replies Last Post
Help! My 3 day old terrarium already has mold :( DesignerofBeauty Terrarium Gardening 9 05-15-2018 09:36 PM
Mold, mold mold! It keeps reappearing! I'm not panicking… yet! donguri Pests & Diseases 21 12-14-2014 06:53 PM
Mold in my flask! Lordoftheswarms Advanced Discussion 3 03-20-2012 12:59 PM
Mold in flask vandagirl Pests & Diseases 6 05-18-2011 05:48 PM
Mold in Flask... *Trouble* Pests & Diseases 7 10-05-2008 02:41 PM

All times are GMT -4. The time now is 02:13 PM.

© 2007 OrchidBoard.com
Search Engine Optimisation provided by DragonByte SEO v2.0.37 (Lite) - vBulletin Mods & Addons Copyright © 2018 DragonByte Technologies Ltd.
Feedback Buttons provided by Advanced Post Thanks / Like (Lite) - vBulletin Mods & Addons Copyright © 2018 DragonByte Technologies Ltd.

Clubs vBulletin Plugins by Drive Thru Online, Inc.