autoclaving fail?
Login
User Name
Password   


Registration is FREE. Click to become a member of OrchidBoard community
(You're NOT logged in)

menu menu

Sponsor
Donate Now
and become
Forum Supporter.

autoclaving fail?
Many perks!
<...more...>


Sponsor
 

Google


Fauna Top Sites
LOG IN/REGISTER TO CLOSE THIS ADVERTISEMENT
  #1  
Old 02-19-2018, 06:56 PM
seagull seagull is offline
Member
 

Join Date: Nov 2017
Zone: 6b
Location: Kingston, RI
Posts: 64
autoclaving fail?
Default autoclaving fail?

so i went to make the banana formulation media from a thread here that had the banana, water, vitamin B1, orchid fertilizer, honey, activated charcoal, and agar
after i did this i poured into jars and pressure cooked for a half hour
3 hours later the gel is hardened but the media has a black precipitate on the bottom and a whitish top layer
would enough nutrients be in that top layer for the seeds or should i chuck the media and retry??
Reply With Quote
  #2  
Old 02-21-2018, 10:46 AM
dansyr dansyr is offline
Member
 

Join Date: Jul 2017
Zone: 6b
Location: Boston
Posts: 54
autoclaving fail? Male
Default

What happened is probably the media is fairly homogenous in terms of nutrients/vitamins, and it's just the charcoal that settled out as it cooled. Happens to me sometimes. But the charcoal is very nice to have evenly mixed as it will come in handy to neutralize some exudates to save you from needing to replate unnecessarily.

A good practice is to gently swirl media as it cools, to keep things distributed. Best practice is to make sure things are fully dissolved at the start But charcoal will usually always settle as it can never really dissolve.

But for now, if your vessels allow, you should be able to reheat the media to melt it (obviously make sure to keep them sterile), and then just make sure you swirl before it sets again.
Reply With Quote
  #3  
Old 02-21-2018, 12:55 PM
seagull seagull is offline
Member
 

Join Date: Nov 2017
Zone: 6b
Location: Kingston, RI
Posts: 64
autoclaving fail?
Default

Quote:
Originally Posted by dansyr View Post
What happened is probably the media is fairly homogenous in terms of nutrients/vitamins, and it's just the charcoal that settled out as it cooled. Happens to me sometimes. But the charcoal is very nice to have evenly mixed as it will come in handy to neutralize some exudates to save you from needing to replate unnecessarily.

A good practice is to gently swirl media as it cools, to keep things distributed. Best practice is to make sure things are fully dissolved at the start But charcoal will usually always settle as it can never really dissolve.

But for now, if your vessels allow, you should be able to reheat the media to melt it (obviously make sure to keep them sterile), and then just make sure you swirl before it sets again.
i reheated and swirled
it's much better
Reply With Quote
Post Thanks / Like - 1 Likes
Likes dansyr liked this post
Reply

Bookmarks

Tags
banana, hardened, layer, media, top


Currently Active Users Viewing This Thread: 1 (0 members and 1 guests)
 
Thread Tools
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off


Similar Threads
Thread Thread Starter Forum Replies Last Post
Grrr! Plant shipment FAIL modern_fumie Beginner Discussion 22 08-17-2015 11:49 AM
More Obamacare Fail Laserbeak Off Topic - Totally 2 12-17-2013 08:35 AM
Catt amethystoglossa fail isurus79 Semi-Hydroponic Culture 20 03-08-2013 05:50 PM
Amaryllis Fail Melody Off Topic - Totally 1 01-03-2011 02:24 PM
Paph blooms fail to open completely josterha Cypripedium Alliance - Paphiopedilum 8 07-23-2010 03:54 AM

All times are GMT -4. The time now is 02:00 AM.

© 2007 OrchidBoard.com
Search Engine Optimisation provided by DragonByte SEO v2.0.37 (Lite) - vBulletin Mods & Addons Copyright © 2024 DragonByte Technologies Ltd.
Feedback Buttons provided by Advanced Post Thanks / Like (Lite) - vBulletin Mods & Addons Copyright © 2024 DragonByte Technologies Ltd.

Clubs vBulletin Plugins by Drive Thru Online, Inc.