Adjust pH of agar????
Login
User Name
Password   


Registration is FREE. Click to become a member of OrchidBoard community
(You're NOT logged in)

menu menu

Sponsor
Donate Now
and become
Forum Supporter.

Adjust pH of agar????
Many perks!
<...more...>


Sponsor
 

Google


Fauna Top Sites
LOG IN/REGISTER TO CLOSE THIS ADVERTISEMENT
  #1  
Old 11-01-2017, 09:40 PM
shmohney shmohney is offline
Jr. Member
 

Join Date: Nov 2017
Posts: 2
Adjust pH of agar????
Default Adjust pH of agar????

Total noob here. Basic question is, do I need to adjust the pH of agar when flasking seeds, and if so, how and to what pH? Details - I have a couple of pink and yellow ladyslipper pods from plants that grow on my property in central Virginia USA. I plan to use distilled water with T849 with coconut water, and M551 with sucrose. I can't seem to find out much about this pH question when kicking around this forum. Details about me - I love native plants and enjoy growing them on my place. In the last couple of years I also started the hobby of cloning and growing local and gourmet mushrooms, raising them in sawdust bags in my basement. Growing mushrooms turns out to use most of the same equipment and skills that propagating orchids from seed uses. Anyway, I'd greatly appreciate any advice I can get here - thanks!
Reply With Quote
  #2  
Old 11-02-2017, 10:02 AM
PaphMadMan PaphMadMan is offline
Senior Member
 

Join Date: Sep 2009
Zone: 5a
Location: Madison WI
Age: 58
Posts: 2,367
Adjust pH of agar???? Male
Default

Media formulations are generally buffered to a specific range - 4.25 0.5 for M551 and 5.25 0.5 for T849, according to the Phytotech website. Other sources might vary somewhat. They may come with a certificate of analysis that tells what pH was found in testing. Unless you have an additive with a very strong pH or buffering of its own the buffering of the media should hold.

You can prepare the media and test/adjust pH before dispensing and sterilizing. Most often 1N HCl or 1N NaOH would be used to adjust pH, or somewhat more or less dilute solutions would obvious also work. What pH you want for your use is harder to say, and I'm sure there are divergent recommendations. Since both media are intended for terrestrial orchids they are probably reasonable for your use as they are. Even better if you can test them and confirm the pH so at least you know what you have as a basis for future adjustments based on success.
Reply With Quote
  #3  
Old 11-06-2017, 12:34 PM
newflasker newflasker is offline
Senior Member
 

Join Date: Jun 2007
Location: Virginia
Posts: 154
Adjust pH of agar????
Default

It's better to adjust pH between 5.5 - 5.8 in tissue culture. For terrestrial orchids, some prefer Terrestrial Orchid Medium w/out NH4NO3.

See the following link for more information:
About Orchids
Reply With Quote
Reply

Bookmarks

Tags
adjust, couple, details, growing, water


Currently Active Users Viewing This Thread: 1 (0 members and 1 guests)
 
Thread Tools
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off


Similar Threads
Thread Thread Starter Forum Replies Last Post
The best homemade orchid flasking medium Mr. Tech Propagation 21 10-13-2016 06:44 PM
How strong does the agar have to be? Tindomul Propagation 21 01-28-2011 06:23 AM
dendrobium agar medium ludisia29 Propagation 6 11-11-2009 10:21 AM
Do you adjust pH when watering? newflasker Advanced Discussion 78 12-06-2007 09:20 AM
compoting....agar on or agar off?? Marco Beginner Discussion 8 11-03-2006 09:21 PM

All times are GMT -4. The time now is 01:25 PM.

© 2007 OrchidBoard.com
Search Engine Optimisation provided by DragonByte SEO v2.0.37 (Lite) - vBulletin Mods & Addons Copyright © 2017 DragonByte Technologies Ltd.
Feedback Buttons provided by Advanced Post Thanks / Like (Lite) - vBulletin Mods & Addons Copyright © 2017 DragonByte Technologies Ltd.

Clubs vBulletin Plugins by Drive Thru Online, Inc.