Question about replating and hydrogen peroxide

[Silje]

Originally Posted by Orchid Whisperer

Hi Silje

I have used this non-glove box method, which relies on chlorine bleach:
Very Easy Sterilisation Method - My Seedlings

I have not replated, but instead have had modest success moving very small protocorms onto fine chopped sphagnum which was boiled and cooled first. The tiny plants develop very slowly, but I am not trying to produce huge numbers of plants quickly.

I've found my germination method. 'The lazy way' works perfect for me and requires so little, I'm going to stick with it. This is more for the replating.

What I was considering was to just pick out some of the protocorms/seedlings in the the densest flasks and then just sterilize the flask with H2O2 again. Which would be super easy. I'm lazy as you can tell.

But I have no idea if seedlings can handle H2O2.

---------- Post added at 06:47 PM ---------- Previous post was at 06:43 PM ----------

Originally Posted by Subrosa

I cannot speak to orchids, but I recently germinated some seeds of a mesemb known for damping off if you think about looking at them wrong. I watered with household H2O2 at full strength (3%) and had no issues with fungi or seedling damage.

Cool! That sounds promising. I've normally used 5% H2O2 for sowing, but I can weaken it a bit.

Thank you!