Originally Posted by adriponic
Mother flasks (inclusive of tissue culture) 1,457
Mother flasks contaminated 36 = 2.5%
Replate flasks = 8,613
Replates contaminated = 69 = 4.75%
I work in a sterile room cleaned with a laminar flow 30 mins ahead of operations which are carried in a sterile box.
WOW, 1,457 mother flasks + 8,613 relate flasks + Tissue Culture = flask Master
. What kind of Tissue Culture have you been working with? I have no problem with orchid seed mother flask but with replate flask. The problem happens when I transfer seedlings from one jar to another jar. I think I need to use Clorox/NaDCC but I hate chlorine odor.