Very Easy Sterilisation Method - My Seedlings
Login
User Name
Password   


Registration is FREE. Click to become a member of OrchidBoard community
(You're NOT logged in)

menu menu

Sponsor
Donate Now
and become
Forum Supporter.

Very Easy Sterilisation Method - My Seedlings
Many perks!
<...more...>


Sponsor
 

Google


Fauna Top Sites
LOG IN/REGISTER TO CLOSE THIS ADVERTISEMENT
  #1  
Old 02-01-2011, 05:28 PM
roby roby is offline
Senior Member
 

Join Date: Nov 2010
Member of:AMAO
Location: Naples, IT
Posts: 305
Very Easy Sterilisation Method - My Seedlings Male
Cool Very Easy Sterilisation Method - My Seedlings



This is my seeding in homemade media, with gaseous sterilization system (sodium hypochlorite fumes)


Epidendrum radicans



Epidendrum radicans replating with fresh medium



Sophronitis coccinea



Chysis aurea replating with fresh medium



Bletilla striata alba


In replating fresh medium I'm trying a supplement based on amino acids and short chain peptides (Siapton L10)

See how it works
Reply With Quote
Post Thanks / Like - 17 Likes
  #2  
Old 02-02-2011, 05:58 AM
Jeff9 Jeff9 is offline
Senior Member
 

Join Date: Sep 2009
Posts: 248
Very Easy Sterilisation Method - My Seedlings
Default

Looks amazing, can you tell us about the gaseous sterilization?
Reply With Quote
Post Thanks / Like - 1 Likes
Likes oak-orchids liked this post
  #3  
Old 02-02-2011, 07:04 AM
roby roby is offline
Senior Member
 

Join Date: Nov 2010
Member of:AMAO
Location: Naples, IT
Posts: 305
Very Easy Sterilisation Method - My Seedlings Male
Default

I do not know if I can explain in English, but I try

is a system that prevents the sterilization of seeds and can work in non-sterile environment

I got the idea from this Protocol Brazilian http://www.scielo.br/pdf/hb/v24n2/19.pdf
Protocol explain how to disinfect the seeds with sodium hypochlorite fumes

I have adjusted the system to my needs

Prepare a sheet of plastic wrap with a hole


create an absorbent pad soaked in sodium hypochlorite


Sow without a sterile box




place the sheet with the hole on the jar and position the absorbent pad soaked in sodium hypochlorite


Cover with a sheet of plastic wrap without hole


just close



after 3 - 4 hours remove the absorbent pad by slightly raising the sheet.



close properly


cut the excess plastic


contamination 10% of jar (40 jar, 4 contamination)

I apologize for my English
Reply With Quote
Post Thanks / Like - 7 Likes
  #4  
Old 02-02-2011, 07:27 AM
Phalguy Phalguy is offline
Senior Member
 

Join Date: Apr 2006
Zone: 5a
Location: Montreal, Canada
Age: 64
Posts: 325
Default

Hello!

If I'm correct, you sow the seeds without any sterilization, then you place a plastic ( with a hole) on top, cover with a cotton pad saturated with bleach for a few hours, then remove the pad and cover right?
And you only got 10% contamination that way???
Reply With Quote
Post Thanks / Like - 3 Likes
Likes neophyte, gerneveyn, Chemtiger liked this post
  #5  
Old 02-02-2011, 08:07 AM
roby roby is offline
Senior Member
 

Join Date: Nov 2010
Member of:AMAO
Location: Naples, IT
Posts: 305
Very Easy Sterilisation Method - My Seedlings Male
Default

Yes is correct
no more than 5 hour, high time to fumes exposition kills seeds, or turn brown leaf of the young plant
Reply With Quote
  #6  
Old 02-02-2011, 08:39 AM
camille1585's Avatar
camille1585 camille1585 is offline
Administrator
 

Join Date: Oct 2007
Location: middle of the Netherlands
Posts: 13,767
Default

I have never heard of that method of sterilization before, but it sounds worth a try! I'm very very surprised that you only get 10% contamination with this, that's amazing. I have some seed to flask, and I think I may try to flask some with this method, it sounds much easier than the 'normal' way of doing it.

Do you sterilize the the medium beforehand (pressure cooker for example?) Do you disinfect the seeds before or is the bleach enough to do it?

And would you mind sharing your homemade medium recipe?

Don't worry about your English, it's quite good and I had no trouble understanding!
__________________
Camille

Completely orchid obsessed and loving every minute of it....

My Orchid Photos
Reply With Quote
  #7  
Old 02-02-2011, 08:52 AM
Daethen Daethen is offline
Senior Member
 

Join Date: Sep 2010
Zone: 4a
Location: Wisconsin, USA
Posts: 2,215
Very Easy Sterilisation Method - My Seedlings Female
Default

This is very interesting. It would make flasking easy for all of us! Your english is just fine.
Reply With Quote
Post Thanks / Like - 1 Likes
Likes cday2inflorida liked this post
  #8  
Old 02-02-2011, 09:12 AM
Royal Royal is offline
Senior Member
 

Join Date: Jan 2008
Zone: 8a
Location: Piney Woods of East Texas
Age: 46
Posts: 3,253
Default

That's excellent. Thank you for sharing this info!
Reply With Quote
  #9  
Old 02-02-2011, 10:58 AM
Lady Tottington Lady Tottington is offline
Senior Member
 

Join Date: May 2010
Zone: 4b
Location: Logan, Utah
Posts: 396
Very Easy Sterilisation Method - My Seedlings Female
Default

I am interested in knowing your recipe for medium as well. AND, I agree that your English is great!!! I totally understood everything, and I really like the idea of trying your methods! Thanks for sharing, and thanks for sharing the photo tutorial, it totally makes sense. I like having pictures so I know how to replicate

I've never flasked, etc. before, but would like to try!
Reply With Quote
Post Thanks / Like - 1 Likes
Likes orchiddream liked this post
  #10  
Old 02-02-2011, 02:15 PM
roby roby is offline
Senior Member
 

Join Date: Nov 2010
Member of:AMAO
Location: Naples, IT
Posts: 305
Very Easy Sterilisation Method - My Seedlings Male
Default

Quote:
Originally Posted by camille1585 View Post
Do you sterilize the the medium beforehand (pressure cooker for example?)
oven (150 ° C 25 minutes)

not disinfect the seeds, in fact if you go over 5 hours die fungi, bacteria and seeds

my medium is simple:

1 L RO water
1.5 g 20-20-20 NPK (Peters formula)
20 g sugar
1 g activated charcoal
5 drops NAA
5 g Agar

PH 5.8

This medium does not work with Catleya, aerangis, spathoglottis

has been tested with
Bletilla
Phalaenopsis
Doritis
Dendrobium
Sophronitis
Restrepia
Masdevallia


I'm happy if you try

There are few rules
clean all tools
do not put head and hands over the jar
prepare the absorbent pad on a blotting paper soaked in alcohol to prevent the electrostatic action of plastic. (picture 1 and 2)

I'd like to know the results
Reply With Quote
Post Thanks / Like - 3 Likes
Likes Ashley9, OrchidSkorpion, Goce liked this post
Reply

Bookmarks

Tags
epidendrum, fresh, medium, radicans, replating, seedlings, method, easy, sterilisation


Currently Active Users Viewing This Thread: 1 (0 members and 1 guests)
 
Thread Tools
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off


Similar Threads
Thread Thread Starter Forum Replies Last Post
My Phal potting mix, watering regime & method OzPhal Potting & Repotting 11 08-04-2012 08:00 AM
Feeding Seedlings? Shere Crossman Beginner Discussion 6 10-29-2009 12:21 AM
Reflasking more mature phal seedlings - advice needed s1214215 Propagation 11 04-24-2009 11:22 AM
Vanda seedlings saved...I hope! dgenovese1 Propagation 11 10-30-2008 09:53 AM
The Cattleya "Cut-Divide-and-Conquer" Multiplier Method Slipperhead Propagation 15 09-08-2007 08:35 AM

All times are GMT -4. The time now is 12:22 PM.

© 2007 OrchidBoard.com
Search Engine Optimisation provided by DragonByte SEO v2.0.37 (Lite) - vBulletin Mods & Addons Copyright © 2024 DragonByte Technologies Ltd.
Feedback Buttons provided by Advanced Post Thanks / Like (Lite) - vBulletin Mods & Addons Copyright © 2024 DragonByte Technologies Ltd.

Clubs vBulletin Plugins by Drive Thru Online, Inc.