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02-01-2011, 05:28 PM
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Join Date: Nov 2010
Location: Naples, IT
Posts: 305
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Very Easy Sterilisation Method - My Seedlings
This is my seeding in homemade media, with gaseous sterilization system (sodium hypochlorite fumes)
Epidendrum radicans
Epidendrum radicans replating with fresh medium
Sophronitis coccinea
Chysis aurea replating with fresh medium
Bletilla striata alba
In replating fresh medium I'm trying a supplement based on amino acids and short chain peptides (Siapton L10)
See how it works
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cday2inflorida, King_of_orchid_growing:), GardenTheater, lvanderb, dendro king, Stray59, escualida, Viper, orchiddream, NYCorchidman, Tetouan, Robe, Abel-Purete, ThePinkCucumber, OrchidSkorpion, oak-orchids, dnatural1 liked this post
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02-02-2011, 05:58 AM
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Looks amazing, can you tell us about the gaseous sterilization?
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02-02-2011, 07:04 AM
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Join Date: Nov 2010
Location: Naples, IT
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I do not know if I can explain in English, but I try
is a system that prevents the sterilization of seeds and can work in non-sterile environment
I got the idea from this Protocol Brazilian http://www.scielo.br/pdf/hb/v24n2/19.pdf
Protocol explain how to disinfect the seeds with sodium hypochlorite fumes
I have adjusted the system to my needs
Prepare a sheet of plastic wrap with a hole
create an absorbent pad soaked in sodium hypochlorite
Sow without a sterile box
place the sheet with the hole on the jar and position the absorbent pad soaked in sodium hypochlorite
Cover with a sheet of plastic wrap without hole
just close
after 3 - 4 hours remove the absorbent pad by slightly raising the sheet.
close properly
cut the excess plastic
contamination 10% of jar (40 jar, 4 contamination)
I apologize for my English
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02-02-2011, 07:27 AM
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Join Date: Apr 2006
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Location: Montreal, Canada
Age: 64
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Hello!
If I'm correct, you sow the seeds without any sterilization, then you place a plastic ( with a hole) on top, cover with a cotton pad saturated with bleach for a few hours, then remove the pad and cover right?
And you only got 10% contamination that way???
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02-02-2011, 08:07 AM
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Join Date: Nov 2010
Location: Naples, IT
Posts: 305
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Yes is correct
no more than 5 hour, high time to fumes exposition kills seeds, or turn brown leaf of the young plant
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02-02-2011, 08:39 AM
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Join Date: Oct 2007
Location: middle of the Netherlands
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I have never heard of that method of sterilization before, but it sounds worth a try! I'm very very surprised that you only get 10% contamination with this, that's amazing. I have some seed to flask, and I think I may try to flask some with this method, it sounds much easier than the 'normal' way of doing it.
Do you sterilize the the medium beforehand (pressure cooker for example?) Do you disinfect the seeds before or is the bleach enough to do it?
And would you mind sharing your homemade medium recipe?
Don't worry about your English, it's quite good and I had no trouble understanding!
__________________
Camille
Completely orchid obsessed and loving every minute of it....
My Orchid Photos
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02-02-2011, 08:52 AM
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Location: Wisconsin, USA
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This is very interesting. It would make flasking easy for all of us! Your english is just fine.
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02-02-2011, 09:12 AM
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Join Date: Jan 2008
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Location: Piney Woods of East Texas
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That's excellent. Thank you for sharing this info!
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02-02-2011, 10:58 AM
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Join Date: May 2010
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Location: Logan, Utah
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I am interested in knowing your recipe for medium as well. AND, I agree that your English is great!!! I totally understood everything, and I really like the idea of trying your methods! Thanks for sharing, and thanks for sharing the photo tutorial, it totally makes sense. I like having pictures so I know how to replicate
I've never flasked, etc. before, but would like to try!
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02-02-2011, 02:15 PM
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Join Date: Nov 2010
Location: Naples, IT
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Quote:
Originally Posted by camille1585
Do you sterilize the the medium beforehand (pressure cooker for example?)
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oven (150 ° C 25 minutes)
not disinfect the seeds, in fact if you go over 5 hours die fungi, bacteria and seeds
my medium is simple:
1 L RO water
1.5 g 20-20-20 NPK (Peters formula)
20 g sugar
1 g activated charcoal
5 drops NAA
5 g Agar
PH 5.8
This medium does not work with Catleya, aerangis, spathoglottis
has been tested with
Bletilla
Phalaenopsis
Doritis
Dendrobium
Sophronitis
Restrepia
Masdevallia
I'm happy if you try
There are few rules
clean all tools
do not put head and hands over the jar
prepare the absorbent pad on a blotting paper soaked in alcohol to prevent the electrostatic action of plastic. (picture 1 and 2)
I'd like to know the results
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