Very Easy Sterilisation Method - My Seedlings
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  #1  
Old 02-01-2011, 06:28 PM
roby roby is offline
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Very Easy Sterilisation Method - My Seedlings Male
Cool Very Easy Sterilisation Method - My Seedlings



This is my seeding in homemade media, with gaseous sterilization system (sodium hypochlorite fumes)


Epidendrum radicans



Epidendrum radicans replating with fresh medium



Sophronitis coccinea



Chysis aurea replating with fresh medium



Bletilla striata alba


In replating fresh medium I'm trying a supplement based on amino acids and short chain peptides (Siapton L10)

See how it works
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  #2  
Old 02-02-2011, 06:58 AM
Jeff9 Jeff9 is offline
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Looks amazing, can you tell us about the gaseous sterilization?
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Old 02-02-2011, 08:04 AM
roby roby is offline
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I do not know if I can explain in English, but I try

is a system that prevents the sterilization of seeds and can work in non-sterile environment

I got the idea from this Protocol Brazilian http://www.scielo.br/pdf/hb/v24n2/19.pdf
Protocol explain how to disinfect the seeds with sodium hypochlorite fumes

I have adjusted the system to my needs

Prepare a sheet of plastic wrap with a hole


create an absorbent pad soaked in sodium hypochlorite


Sow without a sterile box




place the sheet with the hole on the jar and position the absorbent pad soaked in sodium hypochlorite


Cover with a sheet of plastic wrap without hole


just close



after 3 - 4 hours remove the absorbent pad by slightly raising the sheet.



close properly


cut the excess plastic


contamination 10% of jar (40 jar, 4 contamination)

I apologize for my English
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Old 02-02-2011, 08:27 AM
Phalguy Phalguy is offline
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Hello!

If I'm correct, you sow the seeds without any sterilization, then you place a plastic ( with a hole) on top, cover with a cotton pad saturated with bleach for a few hours, then remove the pad and cover right?
And you only got 10% contamination that way???
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Old 02-02-2011, 09:07 AM
roby roby is offline
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Yes is correct
no more than 5 hour, high time to fumes exposition kills seeds, or turn brown leaf of the young plant
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  #6  
Old 02-02-2011, 09:39 AM
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camille1585 camille1585 is offline
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I have never heard of that method of sterilization before, but it sounds worth a try! I'm very very surprised that you only get 10% contamination with this, that's amazing. I have some seed to flask, and I think I may try to flask some with this method, it sounds much easier than the 'normal' way of doing it.

Do you sterilize the the medium beforehand (pressure cooker for example?) Do you disinfect the seeds before or is the bleach enough to do it?

And would you mind sharing your homemade medium recipe?

Don't worry about your English, it's quite good and I had no trouble understanding!
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Old 02-02-2011, 09:52 AM
Daethen Daethen is offline
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This is very interesting. It would make flasking easy for all of us! Your english is just fine.
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Old 02-02-2011, 10:12 AM
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That's excellent. Thank you for sharing this info!
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Old 02-02-2011, 11:58 AM
Lady Tottington Lady Tottington is offline
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I am interested in knowing your recipe for medium as well. AND, I agree that your English is great!!! I totally understood everything, and I really like the idea of trying your methods! Thanks for sharing, and thanks for sharing the photo tutorial, it totally makes sense. I like having pictures so I know how to replicate

I've never flasked, etc. before, but would like to try!
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Old 02-02-2011, 03:15 PM
roby roby is offline
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Quote:
Originally Posted by camille1585 View Post
Do you sterilize the the medium beforehand (pressure cooker for example?)
oven (150 C 25 minutes)

not disinfect the seeds, in fact if you go over 5 hours die fungi, bacteria and seeds

my medium is simple:

1 L RO water
1.5 g 20-20-20 NPK (Peters formula)
20 g sugar
1 g activated charcoal
5 drops NAA
5 g Agar

PH 5.8

This medium does not work with Catleya, aerangis, spathoglottis

has been tested with
Bletilla
Phalaenopsis
Doritis
Dendrobium
Sophronitis
Restrepia
Masdevallia


I'm happy if you try

There are few rules
clean all tools
do not put head and hands over the jar
prepare the absorbent pad on a blotting paper soaked in alcohol to prevent the electrostatic action of plastic. (picture 1 and 2)

I'd like to know the results
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epidendrum, fresh, medium, radicans, replating, seedlings, method, easy, sterilisation


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