Very Easy Sterilisation Method - My Seedlings
Login
User Name
Password   


Registration is FREE. Click to become a member of OrchidBoard community
(You're NOT logged in)

menu menu

Sponsor
Donate Now
and become
Forum Supporter.

Very Easy Sterilisation Method - My Seedlings
Many perks!
<...more...>


Sponsor
 

Google


Fauna Top Sites
LOG IN/REGISTER TO CLOSE THIS ADVERTISEMENT
  #81  
Old 06-01-2012, 05:51 AM
NatalieS NatalieS is offline
Senior Member
 

Join Date: Dec 2011
Age: 42
Posts: 614
Very Easy Sterilisation Method - My Seedlings Female
Default

Quote:
Originally Posted by camille1585 View Post
Protocorms can be very slow to grow after germination. Depending on your medi, you may need to replate into something most suited for growing. What sort of light and temp are the flasks getting? They do great under fluorescent lights, temp around 22-24C for ideal growth.
It might very well be that they are just not getting the ideal growing conditions. I don't have the space for a grow-box or even anywhere to fit fluorescent lights, so the flasks are just sitting in the kitchen very near to a south-east facing window. Temps range between 20 and 23C.

As you predicted Camille, my Miltonia spectabilis moreliana has gotten bigger quite quickly so I'll have to move it from my mini greenhouse soon - then I'll move the flasks to there and see if it encourages any more growth.
Reply With Quote
  #82  
Old 06-01-2012, 07:31 AM
camille1585's Avatar
camille1585 camille1585 is offline
Administrator
 

Join Date: Oct 2007
Location: middle of the Netherlands
Posts: 13,683
Default

The light and temp they get now sounds good. Of course they may be doing just fine as it is when you consider that most seedlings stay in a flask for well over a year, so you shouldn't expect them to grow all that fast! I think that if they are staying green and are growing (taking a pic at regular intervals helps to judge)
__________________
Camille

Completely orchid obsessed and loving every minute of it....

My Orchid Photos
Reply With Quote
  #83  
Old 06-01-2012, 09:03 AM
orchidsarefun orchidsarefun is offline
Senior Member
 

Join Date: Oct 2011
Zone: 5b
Location: Chicagoland
Posts: 3,402
Very Easy Sterilisation Method - My Seedlings Male
Default

Quote:
Originally Posted by NatalieS View Post
They are still just very tiny and green. I had absolutely no contamination though. It might be that my home-made media just doesn't have the right mix of nutrients etc. for the seeds/protocorms to develop properly. I don't know how they should look after only two and a bit months though.

I did a cross with two of my phal hybrids to try to get some seed of my own to experiment with. The pod has been developing for a few months now and this morning I noticed it had turned yellow and was starting to split underneath so I quickly removed it and put it in a container. It seems a bit early for this to have happened so I hope it simply hasn't aborted the pod. Anyway, if it looks like there is some seed I will be experimenting with sowing again this weekend. Fun fun fun!
Thanks.....and GL on the phal. The oldest unripe phal capsules I have are from mid-December, I am watching them carefully. As for your dens, you could try replating a couple into a commercial mix if you are wary of lack of growth?
I don't know if you have seen this site....
Lotte & Thomas Orchids

Roby's method is novel....and I just may try it. The more people that push the envelope, the better
Reply With Quote
  #84  
Old 06-01-2012, 09:12 AM
camille1585's Avatar
camille1585 camille1585 is offline
Administrator
 

Join Date: Oct 2007
Location: middle of the Netherlands
Posts: 13,683
Default

I will be trying Roby's method soon. If many of are successful with it, it will make home flasking accessible to many more people!
__________________
Camille

Completely orchid obsessed and loving every minute of it....

My Orchid Photos
Reply With Quote
  #85  
Old 06-01-2012, 11:53 AM
Call_Me_Bob Call_Me_Bob is offline
Senior Member
 

Join Date: Jun 2009
Zone: 6a
Location: Pennsylvania
Age: 28
Posts: 6,061
Very Easy Sterilisation Method - My Seedlings Male
Default

Good luck Camille!

I had contamination in some of mine. Some are fine though. No growth yet though.

How much light should they get?

I'm worried about when I have to reflask them, assuming they grow. I don't know where to get a glove box!
Reply With Quote
  #86  
Old 06-01-2012, 04:32 PM
camille1585's Avatar
camille1585 camille1585 is offline
Administrator
 

Join Date: Oct 2007
Location: middle of the Netherlands
Posts: 13,683
Default

The info on flask care (light temp) I got from a book on flasking from the library. I'd have to look at it again.

You could try to make a glove box with a plastic storage container. Cut 2 holes in the side, attach a plastic sleeve on each and then a rubber glove. And voila!
I'm going to flask 2 different ways: some seed at home with Roby's method, and some at work using the autoclave and the laminar flow cabinet, and store the flasks in our climate cabinets.
__________________
Camille

Completely orchid obsessed and loving every minute of it....

My Orchid Photos
Reply With Quote
  #87  
Old 06-01-2012, 04:51 PM
jaxorchidman jaxorchidman is offline
Senior Member
 

Join Date: Sep 2009
Zone: 9a
Location: NE Florida
Age: 43
Posts: 317
Very Easy Sterilisation Method - My Seedlings Male
Default

I flasked some dendrobium seed back in February. They are now about 1/16 inches tall and have their first set of tiny leaves. I'll post some pics here soon. Maybe tomorrow. I'm really excited about using this technique and plan on using it soon with some Cattleya seeds from a cross I made last December.
Reply With Quote
  #88  
Old 06-02-2012, 08:54 AM
NatalieS NatalieS is offline
Senior Member
 

Join Date: Dec 2011
Age: 42
Posts: 614
Very Easy Sterilisation Method - My Seedlings Female
Default

Quote:
Originally Posted by orchidsarefun View Post
Thanks.....and GL on the phal. The oldest unripe phal capsules I have are from mid-December, I am watching them carefully. As for your dens, you could try replating a couple into a commercial mix if you are wary of lack of growth?
I don't know if you have seen this site....
Lotte & Thomas Orchids

Roby's method is novel....and I just may try it. The more people that push the envelope, the better
Cheers - I do already have that link in my bookmarks. I think I'll give them a month or two more and if I don't see much more growth I'll start planning a replate.
Reply With Quote
  #89  
Old 12-31-2012, 09:30 AM
NatalieS NatalieS is offline
Senior Member
 

Join Date: Dec 2011
Age: 42
Posts: 614
Very Easy Sterilisation Method - My Seedlings Female
Default Update

Unfortunately my bellatulum seeds didn't go far beyond germination. I think I was not maintaining the correct conditions. I am however pleased to report successful germination of some bulbo orectopetalum seeds which I have subsequently replated. All using Roby's vapour sterlisation method. They're about three to four months old now.



Reply With Quote
Post Thanks / Like - 3 Likes
Likes Susie11, roby, Larssh liked this post
  #90  
Old 12-31-2012, 09:40 AM
Susie11 Susie11 is offline
Senior Member
 

Join Date: Jul 2011
Location: London
Age: 50
Posts: 682
Default

Wow, well done that is really cool!!
Reply With Quote
Post Thanks / Like - 1 Likes
Likes NatalieS liked this post
Reply

Bookmarks

Tags
epidendrum, fresh, medium, radicans, replating, seedlings, method, easy, sterilisation


Currently Active Users Viewing This Thread: 5 (0 members and 5 guests)
 
Thread Tools
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off


Similar Threads
Thread Thread Starter Forum Replies Last Post
My Phal potting mix, watering regime & method OzPhal Potting & Repotting 11 08-04-2012 08:00 AM
Feeding Seedlings? Shere Crossman Beginner Discussion 6 10-29-2009 12:21 AM
Reflasking more mature phal seedlings - advice needed s1214215 Propagation 11 04-24-2009 11:22 AM
Vanda seedlings saved...I hope! dgenovese1 Propagation 11 10-30-2008 09:53 AM
The Cattleya "Cut-Divide-and-Conquer" Multiplier Method Slipperhead Propagation 15 09-08-2007 08:35 AM

All times are GMT -4. The time now is 12:52 AM.

© 2007 OrchidBoard.com
Search Engine Optimisation provided by DragonByte SEO v2.0.37 (Lite) - vBulletin Mods & Addons Copyright © 2024 DragonByte Technologies Ltd.
Feedback Buttons provided by Advanced Post Thanks / Like (Lite) - vBulletin Mods & Addons Copyright © 2024 DragonByte Technologies Ltd.

Clubs vBulletin Plugins by Drive Thru Online, Inc.