Very Easy Sterilisation Method - My Seedlings

[Phalguy]

A month ago, I saw 6 flask with Phal tetraspis X Phal violacea on P668 media... following your instruction... so far I got 4 contamination, 2 are germinating.... next time, I will use your method but in a glove box to increase the chance of succes! Thanks! ;-)

[roby]

Thank you for reporting the result.
Remember that the primary source of contamination is our breath, our hair. The glove box is fine.

[roby]

Hi, small update after 3 - 4 months

Sophronitis coccinea


Bletilla striata alba


Epidendrum radicans

[Fernando]

Thats amazing! I've just sowed the traditional way in a glove box, next time I'm trying this method!
Thanks!!! It will be great for replating!

Fer

[roby]

Originally Posted by Fernando

Thats amazing! I've just sowed the traditional way in a glove box, next time I'm trying this method!
Thanks!!! It will be great for replating!

Fer

for replanting, no more than 2.30 hours
avoid browning of some leaves, and the plants will start soon

---------- Post added at 08:55 AM ---------- Previous post was at 08:50 AM ----------

Originally Posted by CymPaphZygo

Very intruiging - i was wondering if you have deflasked any of the plantlets

only a Bletilla ochracea (November) , I left the small bulbs in the fridge until last week, and now is in pots outside, germinates in the spring...I hope

[daniel]

good post Roby

i will try this also
to bad i did not see this post before
i have been doing some den seeds last weekend
if i new this sooner.i could give it a try :-(

but i have a question on your post in the past on your
Pollinate tips
i have been having good success on hand pollinate on all my orchid flower
but there one that will not work
Psychopsis Papilion?

[Fernando]

Just found five flasks with contamination.
I tried this bleach fumes technique, exposing the five flask 30 minutes to fumes. We'll see if it works. If it does, I've a lot of photos to post!
Regards
Fer

[Tsuchibuta]

Originally Posted by Fernando

Just found five flasks with contamination.
I tried this bleach fumes technique, exposing the five flask 30 minutes to fumes. We'll see if it works. If it does, I've a lot of photos to post!
Regards
Fer

So does this technique actually work to decontaminate the flasks as well?

[Phalguy]

[quote=roby;463985]for replanting, no more than 2.30 hours
avoid browning of some leaves, and the plants will start soon

---------- Post added at 08:55 AM ---------- Previous post was at 08:50 AM ----------



Do you mean that we can use the fume technique for replating?

[CymPaphZygo]

Very intruiging - i was wondering if you have deflasked any of the plantlets