Very Easy Sterilisation Method - My Seedlings

[Phalguy]

Originally Posted by Frankco

Pretty cool Roby. Thanks. I haven't heard of this method. I'll have to get Arditti's book one of these days as I see he tested it and suggest that this is a viable method.

For your media, how much NAA is in 5 drops? Have you tried Cattleya without it or lowering the amount?

Hello!

just did a quick search on amazon for joseph Arditi book and it is listed at $$$$ 475.00 can !!

Micropropagation of Orchids, 2 Volume Set: Amazon.ca: Joseph Arditti: Books

[Frankco]

Hehe that's why I haven't gotten yet. I can buy a lot of stuff or waste a lot of stuff for that price. I hear it's worth every penny though. It's huge and covers every aspect of propagating orchids. Aaron Hicks' book is a bit more reasonable.

[roby]

I do not know how 5 drops, I can tell you that the product is a solution of 0.01% NAA.
I have always used this dose (recommended by producer)

[Frankco]

Hi Roby, never mind. You are using very little NAA, about 0.0005mg per liter. That should not be a problem.

Thank you Roby for the nice explaination and picture tutorials to go along with it. I'm interested to see how others do with the method. I will try this on a few other things besides orchid seeds.

[roby]

Originally Posted by Frankco

Hi Roby, never mind. You are using very little NAA, about 0.0005mg per liter. That should not be a problem.

What would be a most appropriate dose
I try

Thank you

[Mr. Tech]

Hello Mr. Tech
What you sow?

Hello!

I sown Ascocentrum miniatum.

[calypsoB]

beautiful little babies!

[Frankco]

Hi Rody, It depends of the species. With some a little NAA will help in germination. With others it may inhibit germination. Optimal amount? Well, that’s probably in Dr. Arditti’s book too. I’ll leave that question for the experts: -) Just my opinion, I would leave it out for Cattleya unless, others found it to be beneficial.

NAA helps with Vanda. Reference (Mathews and Rao 1980). Helps with Phalaenopsis reference (Baker et al 1987).

Paphiopedilum and Dendrobium NAA is probably not good for germination. Reference (Kano, 1965) and Miyazaki and Nagamatsu (1965). Cleisostoma not good. Reference (Temjensangba adn Chitta Ranjan Deb 2005)

[roby]

Thank you

[Ben Belton]

This is very interesting. Thanks Roby for posting!

I also am interesting in tissue culturing aquatic plants. They are very hard to sterilize without killing them. I am going to try sterilizing them this way.