Very Easy Sterilisation Method - My Seedlings

[Jeff9]

Originally Posted by roby

Hello

I do this technique from May 2010 so I never deflasking, but I think that the transplant in the new medium is fine, so I do not see obstacle to deflasking

I repeated the technique several times, both for improvement is to ensure that it was just a fluke.

In the first replanted, I killed the plants several times to find the correct time of exposure

I can only indicate the dates of sowing

photo 1) sown in June 2010

photo 2) sown in June 2010 - December 2010 replanted in new medium

photo 3) sown in May 2010 - replanted in new mediums January 2011

photo 4) sown in May 2010 replanted in new mediums January 2011

photo 5) sown in January 2011

Thanks to the cooperation of the members of my association I could try a good variety of species.
I will try soon with cymbidium, sophrotes (leptotes x sophronitis ), Angraecum.

In fact it may be that some species can not tolerate even small amounts of chlorine and could die.

but we will see this in the future

Thanks for your questions and thank you for your interest, this is useful to improve

Wow they grow that slow? Photo 3 is Sophronitis Coccinea right? Its almost a year old?

How much light do seedlings take to grow optimal, and how do they get light for you? Your lids of your flasks are not transparent, where does the light come from?

[roby]

Yes is the Sophronitis coccinea

slow growth is due to 3 reasons
1) medium home-made
2) I should replant more often
3) Naples is very hot in late spring and summer, the species that prefer the cool stop completely and then return to vegetate in October.
I have cool in summer, otherwise the temperature goes over 35 ° C
Epidendrum radicans is much more vigorous.


14 hours of light
1200 lm Lamp - 6500 ° K - placed at about 20 cm

The lamp is at the center of a box, and jars around the lamp, the light comes from the side

[Mr. Tech]

Anyway, I did this when I wrote about it. Until now there is no sign of both contamination and germination.

[roby]

Hello Mr. Tech
What you sow?

[Frankco]

Pretty cool Roby. Thanks. I haven't heard of this method. I'll have to get Arditti's book one of these days as I see he tested it and suggest that this is a viable method.

For your media, how much NAA is in 5 drops? Have you tried Cattleya without it or lowering the amount?

[Phalguy]

Originally Posted by Frankco

Pretty cool Roby. Thanks. I haven't heard of this method. I'll have to get Arditti's book one of these days as I see he tested it and suggest that this is a viable method.

For your media, how much NAA is in 5 drops? Have you tried Cattleya without it or lowering the amount?

Hello!

just did a quick search on amazon for joseph Arditi book and it is listed at $$$$ 475.00 can !!

Micropropagation of Orchids, 2 Volume Set: Amazon.ca: Joseph Arditti: Books

[Frankco]

Hehe that's why I haven't gotten yet. I can buy a lot of stuff or waste a lot of stuff for that price. I hear it's worth every penny though. It's huge and covers every aspect of propagating orchids. Aaron Hicks' book is a bit more reasonable.

[roby]

I do not know how 5 drops, I can tell you that the product is a solution of 0.01% NAA.
I have always used this dose (recommended by producer)

[Frankco]

Hi Roby, never mind. You are using very little NAA, about 0.0005mg per liter. That should not be a problem.

Thank you Roby for the nice explaination and picture tutorials to go along with it. I'm interested to see how others do with the method. I will try this on a few other things besides orchid seeds.

[roby]

Originally Posted by Frankco

Hi Roby, never mind. You are using very little NAA, about 0.0005mg per liter. That should not be a problem.

What would be a most appropriate dose
I try

Thank you