Very Easy Sterilisation Method - My Seedlings
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Very Easy Sterilisation Method - My Seedlings
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  #21  
Old 02-04-2011, 04:18 AM
roby roby is offline
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Very Easy Sterilisation Method - My Seedlings Male
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Hi

Let me explain with a drawing
the pad is between two sheets of plastic. one with the hole, and one is without a hole.




When you remove the pad, not completely lift the sheet without a hole, but move only a small part of sheet and remove the pad.

The hole diameter is 1.5 or 2 cm

the jar volume is 100 or 250 ml
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  #22  
Old 02-04-2011, 06:16 AM
Mr. Tech Mr. Tech is offline
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I don't believe in completely sealed flasks. Plants need gasous exchange to photosynthesize.

I did the following:

Sown the seeds in a autoclaved jar without a box, then dipped a multilayer gauze pad in Na-hipochlorite and put it on the jar and put a sheet of aluminium foil on the top then hold them with a rubber ring on the jar.

Few hours later I poked some holes on the foil with a needle to promote gasous exchange.

Last edited by Mr. Tech; 02-04-2011 at 06:18 AM..
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  #23  
Old 02-04-2011, 06:31 AM
roby roby is offline
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I can not tell if it is really necessary gas exchange, personally I never did do, but I know that many people use it.
But it is interesting to know what are the results of your method, I feel in the next seeding
Update me thanks.
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  #24  
Old 02-04-2011, 06:50 AM
roby roby is offline
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Quote:
Originally Posted by Jeff9 View Post
.... if you are the first person to use it for this purpose.

I have a question, did you harvest and pollinate the pods yourself? I have attempted to pollinate orchids alot of times but it never succeeded, any tips?

Hello
gaseous sterilization, is not my invention. I just adapted the method to not use the box sterile

I have no tricks about pollination, which species have tried to pollinate?
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  #25  
Old 02-04-2011, 06:13 PM
Lady Tottington Lady Tottington is offline
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Quote:
Originally Posted by roby View Post
Hi

Let me explain with a drawing
LOVE IT! Thank you for this ))))
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  #26  
Old 02-04-2011, 11:27 PM
Fishballs Fishballs is offline
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Amazing! Thanks.
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  #27  
Old 02-07-2011, 02:57 AM
Mr. Tech Mr. Tech is offline
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There is a problem, because you will never be able to get out chlorine gas out of the flask even of a vented flask because chlorine is 3 times heavier than air. That is why chlorine sits down to the medium and the seeds and may kill them.
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  #28  
Old 02-07-2011, 04:12 AM
roby roby is offline
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True if the chlorine would last forever, but after completing his action is no longer active.
is important that the time is not more than 5 hours
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  #29  
Old 02-07-2011, 04:20 AM
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Quote:
Originally Posted by Mr. Tech View Post
There is a problem, because you will never be able to get out chlorine gas out of the flask even of a vented flask because chlorine is 3 times heavier than air. That is why chlorine sits down to the medium and the seeds and may kill them.
Good point. But if you look at the pics of flasks in the first post, those young seedlings look pretty healthy!

So I guess the question to ask is Roby, have you successfully kept flasks until deflasking?
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  #30  
Old 02-07-2011, 06:24 AM
roby roby is offline
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Hello

I do this technique from May 2010 so I never deflasking, but I think that the transplant in the new medium is fine, so I do not see obstacle to deflasking

I repeated the technique several times, both for improvement is to ensure that it was just a fluke.

In the first replanted, I killed the plants several times to find the correct time of exposure

I can only indicate the dates of sowing

photo 1) sown in June 2010

photo 2) sown in June 2010 - December 2010 replanted in new medium

photo 3) sown in May 2010 - replanted in new mediums January 2011

photo 4) sown in May 2010 replanted in new mediums January 2011

photo 5) sown in January 2011

Thanks to the cooperation of the members of my association I could try a good variety of species.
I will try soon with cymbidium, sophrotes (leptotes x sophronitis ), Angraecum.

In fact it may be that some species can not tolerate even small amounts of chlorine and could die.

but we will see this in the future

Thanks for your questions and thank you for your interest, this is useful to improve
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