Very Easy Sterilisation Method - My Seedlings
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Very Easy Sterilisation Method - My Seedlings
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  #11  
Old 02-02-2011, 02:33 PM
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camille1585 camille1585 is offline
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Thanks! I just need to find the NAA, otherwise I have everything else. I have Cattleya, Brassavola and Dendrobium seed, so I guess I'll try to make a flask of Den seeds. But I'm in the netherlands and my seeds are in france, so it will have to wait a few months.
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  #12  
Old 02-03-2011, 04:32 AM
Mr. Tech Mr. Tech is offline
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I tried this method today. Hope the best.
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  #13  
Old 02-03-2011, 05:09 AM
roby roby is offline
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well, update me about the results, positive or negative.

How many hours did you leave the pad



thanks

Last edited by roby; 02-03-2011 at 05:11 AM..
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  #14  
Old 02-03-2011, 06:33 AM
Jeff9 Jeff9 is offline
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Hi Roby,

Thanks for the information, i wish i had some seed to sow id like to attempt this

A question, why do you not also sterilize the seeds? Is it more hassle or will this much sterilization kill the seeds?

Thanks,
Jeff
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  #15  
Old 02-03-2011, 12:27 PM
roby roby is offline
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Hi Jeff
This method is just to disinfect the seeds (see pdf)
Then I adjusted the system for seeding without boxes sterile.
For me, the classic method of disinfection of seeds and sowing is too complicated
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  #16  
Old 02-03-2011, 02:26 PM
Phalguy Phalguy is offline
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Hi Roby...

I'm about to sow some Phals very soon and I will try your method! I'll keep you posted!
Thanks for sharing
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  #17  
Old 02-03-2011, 03:15 PM
roby roby is offline
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Excellent!
The most delicate part is when you take away the pad, just lift the top sheet of plastic
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  #18  
Old 02-03-2011, 06:29 PM
Jeff9 Jeff9 is offline
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Once again thanks alot Roby, this technique could potentially completely change the way we cultivate orchids in vitro - if you are the first person to use it for this purpose.

I have a question, did you harvest and pollinate the pods yourself? I have attempted to pollinate orchids alot of times but it never succeeded, any tips?
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  #19  
Old 02-03-2011, 07:13 PM
Phalguy Phalguy is offline
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Hello Jeff9

I do pollinate and harvest seeds from my phals...
After a few try, I have better chance when:
1. I cross an old flower on a young flower ( same thing as humain no? ;-) )
2. pollinate in the morning
3. Don't remove the pollen on the mother flower
4. If selfing, flowers should be on 2 different spike
5. Orchid can be very stubborn!

Since I do that, I have almost no fail...
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  #20  
Old 02-03-2011, 07:16 PM
Phalguy Phalguy is offline
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Quote:
Originally Posted by roby View Post
Excellent!
The most delicate part is when you take away the pad, just lift the top sheet of plastic
Helo again...

First, what do you mean by that? Second, how big is the hole in the plastic wrap?

thanks! ;-)
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