Very Easy Sterilisation Method - My Seedlings
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  #181  
Old 12-22-2020, 04:13 PM
PeterP PeterP is offline
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An alternative way of using gaseous chlorine---opening and closing the medium jars inside a transparent ziplock bag and adding HCL to the bleach so that chlorine gas is released inside the bags---and therefore with much less risk of contamination is described here. Also read the comments where some extra details are described such as turning upside down the jar inside the bag to let the chlorine gas flow out:


---------- Post added at 03:13 PM ---------- Previous post was at 03:12 PM ----------

An alternative way of using gaseous chlorine---opening and closing the medium jars inside a transparent ziplock bag and adding HCL to the bleach so that chlorine gas is released inside the bags---and therefore with much less risk of contamination is described here. Also read the comments where some extra details are described such as turning upside down the jar inside the bag to let the chlorine gas flow out:
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  #182  
Old 12-22-2020, 06:42 PM
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Quote:
Originally Posted by PeterP View Post
An alternative way of using gaseous chlorine---opening and closing the medium jars inside a transparent ziplock bag and adding HCL to the bleach so that chlorine gas is released inside the bags---and therefore with much less risk of contamination is described here. Also read the comments where some extra details are described such as turning upside down the jar inside the bag to let the chlorine gas flow out:
A word of caution about this method: Chlorine Gas is VERY toxic and can be highly dangerous even in small amounts. Be extremely careful with it.
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  #183  
Old 01-20-2021, 01:41 PM
Tcc85 Tcc85 is offline
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Hmm, interesting. However I couldn't find anything in the comments about turning the jars upside down to release the chlorine? I imagine its heavier than air so it just flows out. But since the volume of the bag is finite I don't see that really working very well. There would still be too much chlorine inside the jar.
I have 2 pods ripening any week now so maybe I'll give that a try!

Anyone else tried this? What are your experiences?

Last edited by Tcc85; 01-20-2021 at 01:43 PM.. Reason: did something wrong with quoting so I just deleted it.
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  #184  
Old 01-20-2021, 02:13 PM
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Mr.Fakename Mr.Fakename is offline
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Honestly, just don't try chlorine gas. Sowing seeds isn't worth risking death or permanent invalidity.

If you choose the "green pod" technique, seeds are sterile and you only need to throw the capsule in bleach.

If you prefer collecting the dry seeds, dilute bleach/hydrogen peroxide will do the trick.

If you need more info I can send you a bunch of resources!
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  #185  
Old 01-20-2021, 04:18 PM
Dusty Ol' Man Dusty Ol' Man is online now
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I work in the petroleum industry. Chlorine, in various forms, is part of my experience. Unless you have very specific training in the safe handling of chlorine, and its gaseous state in particular, DO NOT TRY THIS AT HOME! Chlorine gas in very small amounts can cause irreparable lung damage. Excuse the graphic, but it will cause your lungs to fill with fluid and you will drown. Hazmat teams use fully encapsulating chemical suits when dealing with it. Don't put yourself at risk unnecessarily.
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  #186  
Old 01-20-2021, 06:13 PM
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Thanks for the warning guys. I've tried to work with unopened seed pods before, but I was working at home, over the steam and I had to chuck everything away eventually because of the contamination. That's why I thought of using this method.

I guess I'll just try and use one of the laminar flow cabinets at work. Hope my boss doesn't catch me!

Any tip as to how to prevent later contamination of jars? How do you close yours? I was thinking of wrapping the lids with parafilm or similar.
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  #187  
Old 01-21-2021, 05:20 AM
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I use a mix of cling wrap, 3M micropore tape or nothing at all depending on what I have on hand.

That thread is a (old) sticky, maybe you should start a new one with the rest of your questions!
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