Very Easy Sterilisation Method - My Seedlings
Login
User Name
Password   


Registration is FREE. Click to become a member of OrchidBoard community
(You're NOT logged in)

menu menu

Sponsor
Donate Now
and become
Forum Supporter.

Very Easy Sterilisation Method - My Seedlings
Many perks!
<...more...>


Sponsor
 

Google


Fauna Top Sites
LOG IN/REGISTER TO CLOSE THIS ADVERTISEMENT
  #161  
Old 01-28-2015, 03:30 AM
kg5 kg5 is offline
Senior Member
 

Join Date: Jan 2015
Location: Queensland, Australia
Posts: 244
Very Easy Sterilisation Method - My Seedlings Male
Default

Really good read!

The glove box?

Why not use a large plastic tub with a strong bleach solution 1 inch or 25mm in the bottom and work with the bleach fumes through the whole process?
Reply With Quote
  #162  
Old 03-05-2015, 09:41 PM
jevanh jevanh is offline
Jr. Member
 

Join Date: Mar 2015
Posts: 3
Very Easy Sterilisation Method - My Seedlings
Default

Hi Natalie

How did you clean the flasks? did you autoclave with a pressure cooker as per usual?

Quote:
Originally Posted by NatalieS View Post
My Dendrobium bellatulum seeds have finally germinated! I'm so pleased! Thanks Roby for all your advice!
Reply With Quote
  #163  
Old 03-06-2015, 05:16 AM
proshen's Avatar
proshen proshen is offline
Jr. Member
 

Join Date: Oct 2014
Posts: 14
Very Easy Sterilisation Method - My Seedlings Male
Default update of seed germination

Here is update photo of seed germinatiin of den. pulchellum. i thought i will not replate and let them grow then deflask. would it be okay? Asking experts opinion. though i am happy with my little achivement.
Attached Thumbnails
Very Easy Sterilisation Method - My Seedlings-seed-jpg  
Reply With Quote
Post Thanks / Like - 1 Likes
Likes Lynn in Michigan liked this post
  #164  
Old 04-02-2015, 05:13 PM
Cntry's Avatar
Cntry Cntry is offline
Senior Member
 

Join Date: Jun 2014
Zone: 7b
Location: Baltimore Maryland
Age: 59
Posts: 587
Very Easy Sterilisation Method - My Seedlings Female
Default

bump
Reply With Quote
  #165  
Old 04-02-2015, 05:21 PM
giuseppeorchids's Avatar
giuseppeorchids giuseppeorchids is offline
Member
 

Join Date: Feb 2012
Location: Southern Italy
Age: 18
Posts: 76
Very Easy Sterilisation Method - My Seedlings Male
Default

Quote:
Originally Posted by Fernando View Post
Just tried again with new seed. It's so simple! Lets see if I did work clean.
well, I know that this message is a bit old, but... you've mistaked! you must put the bleach tampon under the lid, not over! I don't know how your flask is now, but if you put the tampon over the lid, it's all perfectly useless!

Last edited by giuseppeorchids; 04-02-2015 at 05:26 PM..
Reply With Quote
  #166  
Old 06-20-2015, 07:39 PM
Colunio Colunio is offline
Jr. Member
 

Join Date: Jun 2015
Posts: 4
Very Easy Sterilisation Method - My Seedlings
Default

Resurrecting an old thread here. What strength % wise is the bleach (sodium hypochlorite) and do you dilute it for use with the pad?
Reply With Quote
  #167  
Old 07-27-2015, 05:57 AM
Fernando Fernando is offline
Senior Member
 

Join Date: Jan 2008
Location: Espaņa
Posts: 429
Very Easy Sterilisation Method - My Seedlings Male
Default

Giuseppe, that is fun!

The cotton pad you see is not soaked; it is the final closing over the tiny hole made for gas exchange!
The chlorine-soaked piece of cotton has long been removed from in between the two layers of plastic wrap under the lid...

Have used this method with many different seeds now, also non-orchid seeds, and contamination is pretty low, always under 10%, but germination is erratic. This can of course be due to many other reasons.

Last edited by Fernando; 07-27-2015 at 06:00 AM..
Reply With Quote
  #168  
Old 02-18-2016, 12:37 AM
Guido Guido is offline
Jr. Member
 

Join Date: Oct 2015
Zone: 10a
Location: Escondido, California
Posts: 24
Very Easy Sterilisation Method - My Seedlings Male
Default

Could you tell me if this procedure can be done on plantlets being transferred from a mother flask to another flask(replaiting). Should this be feasable please provide me with the protocol. Thank you for your time and response to my question. Guido
Reply With Quote
  #169  
Old 06-06-2016, 09:52 AM
NatalieS NatalieS is offline
Senior Member
 

Join Date: Dec 2011
Age: 35
Posts: 614
Very Easy Sterilisation Method - My Seedlings Female
Default

Quote:
Originally Posted by jevanh View Post
Hi Natalie

How did you clean the flasks? did you autoclave with a pressure cooker as per usual?
I know this was asked a looooonnnnngggg time ago, but better late than never I suppose.

I prep my sowing tubs by cleaning them in a solution of Milton and then drying them off in a warm oven. Then I pour the prepared media straight into them, fit the lids loosely, and sterilize in the microwave (800W) at full power for about 8-10mins. Not very conventional, but it works. I've got Bulbophyllum, Oncidium, and Cymbidium seedlings (the Dendrobium didn't grow so well in my first batch of homemade media - trial and error!).
Reply With Quote
  #170  
Old 02-17-2017, 02:02 AM
DMT DMT is offline
Member
 

Join Date: Apr 2015
Location: Kailua-Kona,HI
Posts: 76
Default

I have tried this method it works great and so easy. I kept the pads on for 2 hours 45 mins.
Reply With Quote
Reply

Bookmarks

Tags
epidendrum, fresh, medium, radicans, replating, seedlings, method, easy, sterilisation


Currently Active Users Viewing This Thread: 1 (0 members and 1 guests)
 
Thread Tools
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off


Similar Threads
Thread Thread Starter Forum Replies Last Post
My Phal potting mix, watering regime & method OzPhal Potting & Repotting 11 08-04-2012 08:00 AM
Feeding Seedlings? Shere Crossman Beginner Discussion 6 10-29-2009 12:21 AM
Reflasking more mature phal seedlings - advice needed s1214215 Propagation 11 04-24-2009 11:22 AM
Vanda seedlings saved...I hope! dgenovese1 Propagation 11 10-30-2008 09:53 AM
The Cattleya "Cut-Divide-and-Conquer" Multiplier Method Slipperhead Propagation 15 09-08-2007 08:35 AM

All times are GMT -4. The time now is 05:57 AM.

© 2007 OrchidBoard.com
Search Engine Optimisation provided by DragonByte SEO v2.0.37 (Lite) - vBulletin Mods & Addons Copyright © 2017 DragonByte Technologies Ltd.
Feedback Buttons provided by Advanced Post Thanks / Like (Lite) - vBulletin Mods & Addons Copyright © 2017 DragonByte Technologies Ltd.

Clubs vBulletin Plugins by Drive Thru Online, Inc.