Very Easy Sterilisation Method - My Seedlings
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  #151  
Old 09-09-2014, 07:18 AM
Fernando Fernando is offline
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Very Easy Sterilisation Method - My Seedlings Male
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Just tried again with new seed. It's so simple! Lets see if I did work clean.
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  #152  
Old 09-21-2014, 08:05 AM
Fernando Fernando is offline
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Eleven days now and no contamination. That is very good. But still to early to discearn with naked eye if anything moves.
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  #153  
Old 09-21-2014, 12:08 PM
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No-Pro-mwa No-Pro-mwa is offline
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Good luck.
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  #154  
Old 10-10-2014, 03:52 AM
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proshen proshen is offline
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Default sterilization of homemade media

hi i am new here. please anyone help me how to sterilize germinating media with a microwave oven. As far as i got first need to mix all ingredients of the media,then put needed amount of media in jar, keep the lid of jar little open, put the jar in microwave, set the temp in 150C and keep the oven run for 30 minutes. Am i right?
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  #155  
Old 10-11-2014, 05:26 AM
NatalieS NatalieS is offline
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It sounds like you're mixing up oven sterilisation with microwave sterilisation. If you want to sterilise in an oven, then, then that would be about 150C for 30 minutes, and if you're using plastic containers you'll need to make sure they're oven safe.
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  #156  
Old 10-11-2014, 07:25 AM
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proshen proshen is offline
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thank you NatalieS. Would you please tell me that the process i described is proper order or not? what is the difference between ordinery oven and microwave oven sterilization process?
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  #157  
Old 10-11-2014, 08:18 AM
NatalieS NatalieS is offline
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Well, basically the oven takes longer. In both cases you'd make up your medium over the stove first. Many people have written very good information about the different methods of sterilisation, and I doubt I could improve on the info they've provided. I recommend taking a look at this site as a first step:

Lotte & Thomas Orchids

Lots of info about different sterilisation methods, media preparation, etc.

Also, try searching on YouTube for 'microwave sterilisation of orchid medium'. There are some very good videos there.
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  #158  
Old 10-11-2014, 08:37 AM
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Lot of thanks NatalieS
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  #159  
Old 12-16-2014, 11:43 AM
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please help me with 100% home made media for cattleya / brassavola seed germination. 10 days ago i have sown some den. pulchellum seed in ready made media by Roby 's described method and i am happy still no sign of contamination.only three flask. please pray for success.
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  #160  
Old 01-26-2015, 03:02 PM
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here is my first atempt flask. seeds are turning green now.
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Last edited by proshen; 03-06-2015 at 05:24 AM..
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