Very Easy Sterilisation Method - My Seedlings
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  #121  
Old 04-23-2013, 07:00 AM
omega_z omega_z is offline
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interestibg method. are other types of sterilization agent can be used? such as alcohol, hydrogen peroxide? or it just the one which has volatile properties. thank you.
regards,
yanto
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  #122  
Old 04-25-2013, 07:18 AM
roby roby is offline
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Very Easy Sterilisation Method - My Seedlings Male
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only gaseous agents such as chlorine, can perform this type of sterilization

---------- Post added at 06:18 AM ---------- Previous post was at 06:13 AM ----------

Quote:
Originally Posted by biofuel View Post
Epipactis helleborine have a bigger seeds than other orchids, so I think it's a cause.
Maybe first sterilization seeds in 70% ethanol could solve this problem.
I must try it in this weekend.
sowing with very few seeds, I think there are too many big pieces
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  #123  
Old 08-25-2013, 01:48 AM
Rinzo Rinzo is offline
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Just want to report that i had my first success using this method on the first try! ill post pictures along with details later on once the seedlings are a little more visible since i just was just able to see germination today 8/24/13.
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  #124  
Old 08-25-2013, 02:00 AM
dendro king's Avatar
dendro king dendro king is offline
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Very Easy Sterilisation Method - My Seedlings Male
Question Please help me :p

Quote:
Originally Posted by roby View Post
oven (150 C 25 minutes)

not disinfect the seeds, in fact if you go over 5 hours die fungi, bacteria and seeds

my medium is simple:

1 L RO water
1.5 g 20-20-20 NPK (Peters formula)
20 g sugar
1 g activated charcoal
5 drops NAA
5 g Agar

PH 5.8

This medium does not work with Catleya, aerangis, spathoglottis

has been tested with
Bletilla
Phalaenopsis
Doritis
Dendrobium
Sophronitis
Restrepia
Masdevallia


I'm happy if you try

There are few rules
clean all tools
do not put head and hands over the jar
prepare the absorbent pad on a blotting paper soaked in alcohol to prevent the electrostatic action of plastic. (picture 1 and 2)

I'd like to know the results
Hey guys, would anyone be able to tell me what NAA would mean. Thanks
__________________
Jack


you can get off alcohol, drugs, cigarettes, food and cars... but once you're hooked on orchids you're finished, you never get off orchids, NEVER!
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  #125  
Old 08-27-2013, 10:45 AM
roby roby is offline
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is an Auxin
1-Naphthaleneacetic acid - Wikipedia, the free encyclopedia
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  #126  
Old 08-27-2013, 12:18 PM
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Orchid Whisperer Orchid Whisperer is offline
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I am planning to flask some Cattleya seeds this week.

I have some jars ready that I plan to sew using more conventional methods, but I also want to try this method; I have some extra medium left over, and of course, plenty of seeds.

My medium is home-made, but a different recipe. Google "How to grow orchids from seeds - kitchen style", you will find it.

roby - thank you so much for posting this method!
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  #127  
Old 09-19-2013, 12:32 PM
alaind52 alaind52 is offline
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Hi Roby,
I am new in the "In vitro" sowing and I find the classical method a bit complicated. I have some jars ready for 3 weeks and, so, would like to try the way of sterilization you talked about. I have a solution of sodium hypochl. at 3,6% (12 Chl.). Do you think that the concentration is enough? What level of concentration do you use? Thanks a lot,
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  #128  
Old 10-17-2013, 03:30 PM
Rinzo Rinzo is offline
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Very Easy Sterilisation Method - My Seedlings-20131010_091222-jpgVery Easy Sterilisation Method - My Seedlings-20131010_091234-jpgVery Easy Sterilisation Method - My Seedlings-20131010_091249-jpg
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  #129  
Old 11-09-2013, 07:54 AM
frozzybear frozzybear is offline
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[/COLOR]
Quote:
Originally Posted by Phalguy View Post
Hello Jeff9

I do pollinate and harvest seeds from my phals...
After a few try, I have better chance when:
1. I cross an old flower on a young flower ( same thing as humain no? ;-) )
2. pollinate in the morning
3. Don't remove the pollen on the mother flower
4. If selfing, flowers should be on 2 different spike
5. Orchid can be very stubborn!

Since I do that, I have almost no fail...
Adding my two-cents to the above, which is a GREAT guideline...

If dealing with fragrant orchids (such as Catt's) wait to do pollinating/harvesting of pollen until the flower in question is fragrant (I've had luck with warm days, mid-morning when I begin to notice strong fragrance... skip overcast/cool days)
I'm supposing the plant knows when it is most fertile and sends out the fragrance to attract pollinators.
Also (I would imagine) that nighttime fragrant orchids may be best pollinated at night.

-LVD

Use what talent you possess - the woods would be very silent if no birds sang except those that sang best.
Henry Van Dyke

Last edited by frozzybear; 11-09-2013 at 08:11 AM.. Reason: duplicate
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  #130  
Old 11-13-2013, 11:45 PM
frozzybear frozzybear is offline
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Default Trying it out!

Just completed a series of replates using the gaseous sterilization method described above (with bleach soaked cotton balls for pads, without the use of a hood)... *fingers crossed*

A thought I had while replating:
It seems beneficial to mix a bit less water into the usual agar/nutrient solution so that it firms-up more than usual in the autoclave process.

This should cut down on the amount of plant material that sinks into the agar during replating, thus increasing the amount of plant material exposed to gas exchange for sterilization.

Another wards - a replate plantlet sitting on top of a firm agar surface won't sink.

Flask seedlings being replated:
C. Jose Marti 'Mother's Favorite' x self
This seemed like a perfect opportunity to experiment with this new technique since the flask had around 100 mini seedlings.

(I read about the Jose Marti and Mother's Favorite both being a registered cross of the same parents but registered by different people while the parents had different names, boy oh boy does the naming get confusing!)

-Laura

Use what talent you possess - the woods would be very silent if no birds sang except those that sang best.
Henry Van Dyke
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