Today I try gaseous sterilization.
I used seeds Epipactis helleborine collected in November 2012.
Pad was soaked in 5% NaOCl – 3 hours.
Seeds were sown on modified BM-2 (with 25% coconut water and 10 grams - not 20 - sucrose per liter).
All jars have a lid with a cotton plug.
Medium sterilized for one hour in 180C, electric oven.
I made 6 jars and now I'm waiting for contaminations