Very Easy Sterilisation Method - My Seedlings
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  #111  
Old 02-05-2013, 08:12 PM
Phalguy Phalguy is offline
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Originally Posted by Call_Me_Bob View Post
Nice!!!! How did you sterilize when you replated?

I used the same technique, bleach fume ! On 4 flask, only one got contaminated and 2 dried up over time. It's the only flask surviving so far....

It,s a very long process but soooooooo fun to do !
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  #112  
Old 02-06-2013, 12:51 AM
Call_Me_Bob Call_Me_Bob is offline
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Quote:
Originally Posted by Phalguy View Post
I used the same technique, bleach fume ! On 4 flask, only one got contaminated and 2 dried up over time. It's the only flask surviving so far....

It,s a very long process but soooooooo fun to do !
So you were able to replate with this method as well? Awesome I will have to give it another go! I thought to replate you would still need a glove box or something similar.
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  #113  
Old 02-06-2013, 06:25 PM
Phalguy Phalguy is offline
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I find that using a glove box with this technique minimize the contamination... but you don't have to... I have use the water steam ( on a wok ) instead of glove box with good results..
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  #114  
Old 02-25-2013, 02:42 PM
Call_Me_Bob Call_Me_Bob is offline
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DO you guys think this would work for doing a stem prop? Any thoughts on that?
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  #115  
Old 04-09-2013, 07:48 AM
biofuel biofuel is offline
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Today I try gaseous sterilization.

I used seeds Epipactis helleborine collected in November 2012.
Pad was soaked in 5% NaOCl 3 hours.
Seeds were sown on modified BM-2 (with 25% coconut water and 10 grams - not 20 - sucrose per liter).
All jars have a lid with a cotton plug.
Medium sterilized for one hour in 180C, electric oven.
I made 6 jars and now I'm waiting for contaminations
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Last edited by biofuel; 04-09-2013 at 07:56 AM..
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  #116  
Old 04-09-2013, 07:56 AM
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camille1585 camille1585 is offline
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Your photos are not appearing. Unfortunately you are not allowed to post photos or links until you have made 5 posts. Post a few more times, and then it's good!
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Camille

Completely orchid obsessed and loving every minute of it....

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  #117  
Old 04-09-2013, 08:04 AM
biofuel biofuel is offline
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It's explain why I have a small problems with sharing the photos, thank you
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  #118  
Old 04-19-2013, 03:02 AM
biofuel biofuel is offline
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After ten days I have 8 jars with bacterial contamination...
In two jars I dont see any signs of contamination. I don't know what I did wrong.
I have a suspicion:
Probably under the orchid seeds could be a bacterial spores, and Cl2 can't kill them, because a part of the surface seed coat didn't avaliable to sterilization agent.
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  #119  
Old 04-19-2013, 03:20 AM
escualida escualida is offline
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Quote:
Originally Posted by biofuel View Post
After ten days I have 8 jars with bacterial contamination...
In two jars I dont see any signs of contamination. I don't know what I did wrong.
I have a suspicion:
Probably under the orchid seeds could be a bacterial spores, and Cl2 can't kill them, because a part of the surface seed coat didn't avaliable to sterilization agent.
That makes sense, I wonder if there's a way of getting under there...
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  #120  
Old 04-19-2013, 03:52 AM
biofuel biofuel is offline
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Epipactis helleborine have a bigger seeds than other orchids, so I think it's a cause.
Maybe first sterilization seeds in 70% ethanol could solve this problem.
I must try it in this weekend.
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epidendrum, fresh, medium, radicans, replating, seedlings, method, easy, sterilisation


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