Limiting infection when reflasking - how

[s1214215]

Hi Everyone

I have been using a glove box to reflask and flask seed - how I dream of a laminar flow unit. I still get contamination no matter how careful or over-zealous I am with disinfecting.

I have read a link suggesting the use of hydrogen peroxide when potting seedlings. I wonder, could we use 3% Hydrogen Peroxide when reflasking. I am sure many of you have had flasks sent in the mail, arriving looking like a fruit salad or at best agar all over the leaves. If you dont want to pot up, then you reflask, but that for me has so far been hit and miss.

Another possibility is antibiotics - I just found this link for PPM PPM: A Powerful Tool to Prevent or Eliminate Microbial Contamination in Plant Tissue Culture

Now, some countries prohibit the use of antibiotics in flasks. I also know that some species cant take this particular disinfectant.

How do members here reflask and make sure you dont get infections.


Brett