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  #21  
Old 08-28-2020, 09:08 PM
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Quote:
Originally Posted by Dorchid View Post
That’s the stuff!

Also, what media are you using?
Great, thanks for the advice!
I used p669 for the mother flasks and 80% O156 for the replate, both from OSP on ebay.
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  #22  
Old 08-28-2020, 09:20 PM
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You bet! Sweet...I was looking at that as well as Hill’s. Looks to be working well for you.
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  #23  
Old 08-28-2020, 09:38 PM
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Yeah it worked great! I also picked up some Malmgren's as well for messing around with some terrestrial species I have growing in my yard.
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  #24  
Old 08-28-2020, 09:44 PM
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Waaaay outside my ballpark... but you GO Afid!
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  #25  
Old 08-29-2020, 12:28 PM
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They look awesome!!

I feel like there are more and more people getting into flasking these days! Which is exciting for the future of orchids, especially for species that are endangered or extinct in the wild!
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  #26  
Old 08-29-2020, 02:43 PM
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People can learn the skills on their own, but it's much faster and better to have somebody show you in person. Sterile technique is a complex system of awareness and action. A local community college probably offers a microbiology class with a laboratory session.
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  #27  
Old 08-29-2020, 06:05 PM
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Quote:
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They are not vented unfortunately, I couldn't get my hands on any microfilters that would work. I will upload pics later today.
Simple microporous tape works wonders! The 3M thing is cheap and readily available.
Drill a hole, tape, sterilise, done.
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  #28  
Old 08-29-2020, 06:20 PM
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I’ve used 3M micropore tape in the past but wouldn’t recommend it. If you’re gonna use it it needs to be applied with several layers and replaced with each cycle...honestly it just isn’t that great of a filter. Under a microscope, the spaces between the fibers are massive compared to fungal spores. I know a lot of folks that use a piece of tyvek with mp tape over the top with great success.

Last edited by Dorchid; 08-29-2020 at 06:35 PM..
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  #29  
Old 08-29-2020, 08:24 PM
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Thanks for all the help everyone! I think my second attempt will go much better!

---------- Post added at 05:24 PM ---------- Previous post was at 03:41 PM ----------

Quote:
Originally Posted by Dorchid View Post
I know a lot of folks that use a piece of tyvek with mp tape over the top with great success.
This sounds like an interesting solution, I might try that, since I'm doing small batches it doesn't matter too much if I have to replace the filters a lot.
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  #30  
Old 08-30-2020, 09:16 AM
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Quote:
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Under a microscope, the spaces between the fibers are massive compared to fungal spores.
I've never thought of looking under a microscope, that's actually super smart.
Now I can't help but be paranoid, I'll try your solutions.
Thanks for the heads up!
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