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  #11  
Old 02-15-2020, 04:07 PM
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I do not have that info, but I agree wholeheartedly that there will be generational degradation.
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  #12  
Old 02-15-2020, 04:53 PM
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Quote:
Originally Posted by Subrosa View Post
Ray, would you have any information about the effectiveness of homebrewed Innocucor after several generations? I know from culturing mixed cultures of microalgae that some species tend to predominate over time, while others disappear entirely.
I was thinking along the same lines of some species outcompeting others. That got me wondering about interspecific competition among the species in Innocuor. After applying Innocuor, some species would surely outperform others in terms of survival, and survival rates of each species would vary based on the orchid medium. It might be worthwhile to investigate the environmental requirements of each species of bacterium in Innocuor and figuring out which ones best thrive in specific orchid media (e.g. sphagnum moss, bark, etc.).

Considering that culturing all the species together would lead to some species outcompeting others, a good solution might be to simply figure out a) which species are best for one's own orchids and only using those ones and b) figuring out what cultural conditions that specific species of bacterium needs in vitro. Otherwise, one might end up with a culture predominated by one species of bacterium that ends up dying quickly in the orchid medium. I think that because Innocuor contains the correct proportions of all these bacteria, at least one will end up doing its job in the orchid medium (which is why Inncouor can be used for orchids in various media). But eventually, homebrewed Innocuor might become ineffective because it becomes predominated by the wrong species.

It's probably also a good idea to keep several jars of homebrewed probiotics because one of the jars might get contaminated with a virulent species or horizontal gene transfer might confer one population with toxin genes that would harm orchids.
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  #13  
Old 02-15-2020, 07:59 PM
DirtyCoconuts DirtyCoconuts is offline
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I think it kind of comes down to the law of limiting reagents. If there is abundant food for all the strains then thy would ever compete and there would not be a need for any of them to die off

If in the replicating process there is a shortage of the fuel then youíd have the competition you discuss. But with sufficient sugar they should all make it
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  #14  
Old 02-15-2020, 08:24 PM
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DC, you are right that more abundant sugar means less competition, but there are other factors that affect growth besides the availability of food (and some affect the rate at which the bacteria can acquire food). The species that benefit from these factors (which vary by culture conditions) would crowd out the rest. So even with more sugar, the resources would be split unevenly.
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  #15  
Old 02-15-2020, 09:33 PM
DirtyCoconuts DirtyCoconuts is offline
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I hear you. I wonder, and this is a reckless speculation, if the developers intended that with their careful selection of strains. Perhaps they keep each other in check the way a healthy biological system does.
This often happens with fungal colonies where as one starts to thrive, the waste it produces then fuels the competitiveness of another fungus which reduces the population of the first back to stasis.

?? Maybe but doubtful lol
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  #16  
Old Yesterday, 08:13 AM
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They developed a way to maintain cooperating consortia of microbes.

I know their charge includes molasses, kelp, pure water, salt and clay - some of the species prefer being anchored, rather than swimming, I suppose. Maple syrup was substituted for the molasses when they made some for their own consumption.

When Iíve brewed my own, Iíve used 1:1:1:20+ Inocucor, molasses, KelpMax, RO water.
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Old Yesterday, 10:06 AM
DirtyCoconuts DirtyCoconuts is offline
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Thatís the recipe Iím going with ray.
My garage insulated and air conditioned at stays at 75
I was going to use my new bottle and divide it into four parts and but three in the fridge as future seed bottles. The last quarter will be used for a two week brew and then Iíll jar that whole batch up and put in the garage fridge.

In the fridge how long do you think it will maintain viability?
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  #18  
Old Yesterday, 01:39 PM
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Quote:
Originally Posted by DirtyCoconuts View Post
In the fridge how long do you think it will maintain viability?
You got me...
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