Butterwort Tissue Culture
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  #1  
Old 07-29-2022, 01:12 PM
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Dorchid Dorchid is offline
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Default Butterwort Tissue Culture

Working on propagating Mexican Butterwort via tissue culture. This is Pinguicula Moranensis J. 2 weeks in from sterilized explants (carnivorous leaves and meristem). The leaves have yet to show any signs of striking and the meristem died but the 2 leaves encapsulating the meristem have started to grow. They were whitish pink when I plated them but have now started to unfurl and turn green. Unfortunately, I didnít snap a pic when they were plated. These 2 pics represent 4 days growth between them. Hopefully, they will form calluses and begin to generate plantlets in the near future.
Butterwort Tissue Culture-19f63528-bed7-4803-8ee8-9a2620b73f86-jpgButterwort Tissue Culture-9224ff7d-e0d5-4a7b-add3-aa578e42184a-jpg

Last edited by Dorchid; 07-29-2022 at 06:10 PM..
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Old 08-12-2022, 08:44 PM
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Starting to see some plantlets develop.

Butterwort Tissue Culture-1effa419-d35a-49e0-bc5f-0adf188e195d-jpgButterwort Tissue Culture-e4bc49ca-53ba-4306-af69-b7495cac7778-jpg
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Old 08-13-2022, 05:46 AM
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Butterwort Tissue Culture
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Cool!

So that gel has nutrients as well as growth stimulants and rooting hormones? I guess I don't know much about the nitty gritty of meristem cloning...
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Old 08-13-2022, 02:56 PM
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Yep. This medium is Linsmaier and Skoog basal medium which was developed in 1964. It contains the macro and micronutrients, vitamins and sucrose needed for in-vitro plant development and is kind of an all purpose blend for a variety of plants. Itís too hot for carnivorous plants so the first run was half strength and the second batch was 20% which I just replated to. I also added a couple synthetic hormones/plant growth regulators, BA and NAA in ratios that promoted the most shoot development and plant weight based on a paper I read. Theyíre super potent and only used .2mg/L and .1mg/L of medium, respectively.
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