Definition of Callus versus PLB from Leaf Culture
Login
User Name
Password   


Registration is FREE. Click to become a member of OrchidBoard community
(You're NOT logged in)

menu menu

Sponsor
Donate Now
and become
Forum Supporter.

Definition of Callus versus PLB from Leaf Culture
Many perks!
<...more...>


Sponsor
 

Google


Fauna Top Sites
LOG IN/REGISTER TO CLOSE THIS ADVERTISEMENT
  #1  
Old 02-21-2019, 05:18 PM
Ki6bud's Avatar
Ki6bud Ki6bud is offline
Member
 

Join Date: May 2018
Posts: 47
Default Definition of Callus versus PLB from Leaf Culture

I was reading in a research paper where the author showed two photos and titled the figure, "(A) Callus induction from leaf culture after 8 weeks and (B) PLB induction from leaf culture after 8 weeks." Could some please explain the difference?
Reply With Quote
  #2  
Old 02-22-2019, 04:41 AM
Orchid Whisperer's Avatar
Orchid Whisperer Orchid Whisperer is offline
Senior Member
 

Join Date: Jul 2012
Zone: 7b
Posts: 2,913
Definition of Callus versus PLB from Leaf Culture Male
Default

No idea what PLB is.

Can you somehow direct us to the full article?
__________________
“The good thing about science is that it's true whether or not you believe in it.”

― Neil deGrasse Tyson

"We know the Earth is not flat because, if it was, cats would have pushed everything off the edge of it by now."
Reply With Quote
  #3  
Old 02-22-2019, 10:22 AM
Ki6bud's Avatar
Ki6bud Ki6bud is offline
Member
 

Join Date: May 2018
Posts: 47
Default

PLB is protocorm-like body. Here is the article.

https://www.ajol.info/index.php/ajb/...le/96202/85554

---------- Post added at 08:12 AM ---------- Previous post was at 07:59 AM ----------

From what I have read, a protocorm-like body forms out of callus material. Am I mistaken?

---------- Post added at 08:22 AM ---------- Previous post was at 08:12 AM ----------

By definition protocorms are produced by seeds whereas protocorm like bodies (PLB) are produced by explants.
Reply With Quote
  #4  
Old 02-22-2019, 12:18 PM
neophyte neophyte is offline
Member
 

Join Date: Feb 2016
Location: Bay Area
Posts: 70
Definition of Callus versus PLB from Leaf Culture
Default

I think you are right. A callus is like a mass of undifferentiated cells (analogous to human stem cells). All cells in our bodies have the same DNA, but different tissues express some genes more than others, which is why some cells are liver cells and some are bone cells or lens cells. When undifferentiated cells (stems cells or meristems/calluses) are exposed to certain chemicals, they will differentiate into specific tissues (in plants, they are vascular tissue, leaves, roots, etc) that are determined by what the chemicals/hormones are. So a PLB, I think, is derived from a callus that has started the process of differentiation.


And the reason why not all cells differentiate into the exact same tissues (if they are in the same medium) is because the callus/stem cells will produce the differentiating hormones themselves but concentrate all the hormone on one side of the cell, so when the cell divides, one half will still be a stem cell and the other half will start differentiation.

Last edited by neophyte; 02-22-2019 at 12:21 PM..
Reply With Quote
  #5  
Old 02-22-2019, 01:58 PM
Ki6bud's Avatar
Ki6bud Ki6bud is offline
Member
 

Join Date: May 2018
Posts: 47
Default

Neophyte - that sounds reasonable. Thanks for your input. I'm still in the process of learning all of the terms. Orchid propagation fascinates me.
Reply With Quote
Reply

Bookmarks

Tags
culture, induction, leaf, plb, weeks


Currently Active Users Viewing This Thread: 1 (0 members and 1 guests)
 
Thread Tools
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off


Similar Threads
Thread Thread Starter Forum Replies Last Post
Bean Leaf Neofinetia (Mameba) shakkai Vanda Alliance - Neofinetia 19 08-23-2017 11:59 AM
Oncidium NOID with yellow leaf and black spots on leaf grasshopper Oncidium/Odontoglossum Alliance 1 12-06-2009 10:27 AM
Problems with formosae dendrobiums - yellowing leaf tips calvin_orchidL Dendrobium Alliance 8 08-09-2009 05:38 PM
Novice phal owner - rotten leaf, and crack in another leaf katfemme89 Beginner Discussion 15 01-22-2008 11:07 PM
phal amabilis - lower leaf turning yellow ladyslipper Hybrids 5 05-22-2007 10:07 AM

All times are GMT -4. The time now is 01:57 AM.

© 2007 OrchidBoard.com
Search Engine Optimisation provided by DragonByte SEO v2.0.37 (Lite) - vBulletin Mods & Addons Copyright © 2019 DragonByte Technologies Ltd.
Feedback Buttons provided by Advanced Post Thanks / Like (Lite) - vBulletin Mods & Addons Copyright © 2019 DragonByte Technologies Ltd.

Clubs vBulletin Plugins by Drive Thru Online, Inc.