Are the stomas open?
Login
User Name
Password   


Registration is FREE. Click to become a member of OrchidBoard community
(You're NOT logged in)

menu menu

Sponsor
Donate Now
and become
Forum Supporter.

Are the stomas open?
Many perks!
<...more...>


Sponsor
 

Google


Fauna Top Sites
LOG IN/REGISTER TO CLOSE THIS ADVERTISEMENT
  #1  
Old 08-14-2017, 09:40 PM
dethswatch dethswatch is offline
Jr. Member
 

Join Date: Jan 2016
Posts: 17
Are the stomas open?
Default Are the stomas open?

I've got a microscope (10x, 40, 100), would it be possible to take a sample (with a paper hole punch possibly?) to find out if the stomas are open at any random point throughout the day?

Or are we talking electron microscopy? Or would they quickly close?
-

Also, what would happen if the stomas were kept open (is this possible?) as a consequence of the VPD in the environment- say, 48 hours?
Reply With Quote
  #2  
Old 08-15-2017, 12:19 AM
estación seca's Avatar
estación seca estación seca is offline
Senior Member
 

Join Date: Jun 2015
Zone: 9b
Location: Phoenix AZ - Lower Sonoran Desert
Posts: 17,749
Are the stomas open? Male
Default

Well, stomata were discovered before electron microscopy. Somebody managed to see them with a visible light microscope.

(By the way, singular stoma; plural stomata; from Greek, "mouth")

It's very hard to focus on something more than a few microns thick with a 100x microscope lens. Thicker objects are usually too close to the lens.

I think stomata respond primarily to light and darkness, not relative humidity. I don't know what VPD means. I'm not familiar with experimental literature on keeping them open by manipulating environmental conditions, but I suspect it's been well studied.
__________________
May the bridges I've burned light my way.

Weather forecast for my neighborhood
Reply With Quote
  #3  
Old 08-15-2017, 02:41 PM
naoki naoki is offline
Senior Member
 

Join Date: Apr 2012
Zone: 2a
Location: Fairbanks, AK
Posts: 975
Are the stomas open? Male
Default

Yes, you can see them easily with 10x or 40x objective lens and 10x ocular lens (that's probably what you have). I'm guessing that you are talking about a compound microscope.

You need nail polish and scotch tape. Paint the bottom side of leaf with nail polish (about the size of dime is good enough). After a minute or so (when the polish is dry), you paint the area again to make the coating a thicker. Then you can peel it off by attaching the scotch tape at the edge of the paint. Put it on slide and you can see the imprint.

You can see some of them are open and others closed.

Regulation of stomata is elaborate and controlled by many factors, time of the day (photoperiodism), green and blue light, water stress sugar concentration from photosynthesis etc. One of the best known regulation is abscisic acid (plant hormone) related mechanism. When roots sense that there isn't any water available, they signal it to the leaf by the hormone, and stomata changed ion concentration and close the stomata in response. So vapor pressure deficit is only one of the factors.

I'm not sure if you can keep them open for 48 hours. They usually open and close, and the ratio could be changed in the day time and night time. Why do you want to do it?

Also quite a few species of orchids are CAM. But many of the CAM orchids are facultative. For example, Phalaenopsis behaves like C3 when there is plenty of water around the root, but behaves like CAM when it is drier.
Reply With Quote
  #4  
Old 08-15-2017, 06:24 PM
Orchid Whisperer Orchid Whisperer is offline
Senior Member
 

Join Date: Jul 2012
Zone: 8a
Location: Athens, Georgia, USA
Posts: 3,208
Are the stomas open? Male
Default

When Naoki says nail polish, remember that it is clear nail polish.

You can do roughly the same thing if you have clear acetate film and acetone. Wet a small area on the leaf with acetone, apply the acetate film to the wet spot, let it dry then peel off.

Remember that any efforts to look at stomata will destroy some leaf tissue. Probably don't want to so this too many times.
Reply With Quote
Reply

Bookmarks

Tags
close, happen, microscopy, quickly, stomas


Currently Active Users Viewing This Thread: 1 (0 members and 1 guests)
 
Thread Tools
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off


Similar Threads
Thread Thread Starter Forum Replies Last Post
Sepals open, Petals don't? cegha04 Hybrids 9 04-30-2015 03:16 PM
Two Flowers open together on Paph. liemianum NYCorchidman Cypripedium Alliance - Paphiopedilum 15 05-24-2014 11:10 PM
Santa Barbara Orchid Estate Fall Open House 2010!!! peeweelovesbooks Orchid Show Announcements 0 10-17-2010 02:12 PM
Kaler's open house July 24th and 25th got ants Orchid Show Announcements 12 07-26-2010 08:11 AM
Paph blooms fail to open completely josterha Cypripedium Alliance - Paphiopedilum 8 07-23-2010 03:54 AM

All times are GMT -4. The time now is 09:51 AM.

© 2007 OrchidBoard.com
Search Engine Optimisation provided by DragonByte SEO v2.0.37 (Lite) - vBulletin Mods & Addons Copyright © 2024 DragonByte Technologies Ltd.
Feedback Buttons provided by Advanced Post Thanks / Like (Lite) - vBulletin Mods & Addons Copyright © 2024 DragonByte Technologies Ltd.

Clubs vBulletin Plugins by Drive Thru Online, Inc.