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Old 10-03-2007, 12:51 PM
newflasker newflasker is offline
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Join Date: Jun 2007
Location: Virginia
Posts: 128
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Quote:
Originally Posted by adriponic View Post
Mother flasks (inclusive of tissue culture) 1,457
Mother flasks contaminated 36 = 2.5%
Replate flasks = 8,613
Replates contaminated = 69 = 4.75%

I work in a sterile room cleaned with a laminar flow 30 mins ahead of operations which are carried in a sterile box.
Hi adriponic,
WOW, 1,457 mother flasks + 8,613 relate flasks + Tissue Culture = flask Master . What kind of Tissue Culture have you been working with? I have no problem with orchid seed mother flask but with replate flask. The problem happens when I transfer seedlings from one jar to another jar. I think I need to use Clorox/NaDCC but I hate chlorine odor.
Nguyen
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