Hi Jean Pierre... thanks for hanging in there with me. I have pepared flasks and let them sit for 30-40 days and there hasn't been any contamination so I believe that the problem is when I replate. This last time I had trouble with the sixth and eighth mother flask that I did. So I sit here wondering what I could have done diff between flasks. These flasks came from two diff sources, one in Malaysia and the other from Ecuador. The ones from Malaysia were doubled bagged in sterile conditions and the ones from Ecuador were in the original flasks. The Ecuador ones gave me trouble. I must not be sterilizing the outside of the replate flasks good enough and maybe I touch the edge when I am inserting the plants. I probably will not get any more flasks until spring so I have a lot of time to ponder this and maybe change my procedure. Do you feel the need to vent the replate flasks? It would make things simpler if I didn't.
John
|