Hi Jaysen
1.I do not think it was too early as the pod started to open. Did you notice if the seeds changed color during the
bleach bath? Normally good seeds change to a vivid yellow/
orange color and sink to the bottom of the tube.
2.No. UV radiation stops the unicelluar bacteria from
multiplying. Normally one bacteria at 25 celcius split into
2 every 20 mins or so if some source of energy is present. A load of 1000 cells would reproduce in theory
to a population of 1,073,741,800,000 cells in 10 hours.
But bacteria is not the only contaminators, there are
micro fungii (moulds) as well and I have not found UV to
be very effective against these. Another point to mention here:- UV radiation out of a very limited frequency range will induce skin cancer on repeated
exposure. So you better be careful.
3.I prepare media in bulk and have consistent success
with batches 3 to 6 months old. Important thing is the
flask must be airtight closed to limit drying out of the media.
Hope you find my comments useful
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 Jean Pierre Adrien
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