I agree...the difficulty lies in getting a truly closed system. unless that tank is truly air tight, which I doubt, you're never going to get to 100%. but you can certainly get close!
also, I would think you wouldn't want a truly closed system, because you do want to have some gas exchange. unless, that is, you are going to be piping in fresh gas from a tank, but I don't know if that's a desirable option.
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