Quote:
Originally Posted by Gin
I have not re-plated them , good thing i did the re-plate jars they sat for over a month then grew mold.
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Normally, any latent contamination will surface within 5-10 days. It sounds like incidental contamination after successful sterilization. If it took a month to start showing, I'd check the tightness of your lids and/or double up on the vent closures (maybe on the inside too). Just a thought.
With vents in your lids, pressure is able to equalize as they cool. I normally just autoclave them with the cap loose and foil on top. I tighten the lids while still hot, touching only the foil. I've had zero % contamination in prepared flasks (until I put something in them, then I contaminate about 20%).
